I am culturing Calu-3 on 24-well plate inserts and I always record the measurement for the blank before the wells with cells. However, the reading is not constant for the blank. Some of the articles that I refer to states that the reading for the blank should be 150 Ωcm2 but my reading for blank may even reach 700 Ωcm2. May I know how to fix this problem? Thank you.
Hi Wafda. I'd agree, the blank reading shouldn't vary massively from day-to-day. Also, 700 is very high for a blank - readings are usually in the region of 120 to 150. You may need a new electrode for the TEER instrument. The electrodes are quite easily damaged, and in the past when we've had issues with odd TEER readings, it has usually been the electrode.
Your reading for a blank insert (membrane only) should not fluctuate. Factors that may influence the TEER reading include the buffer used and the volume used (apically and also basolaterally). If you've kept these consistent, i recommend scrutinizing the instrument used to make the readings to ensure it is operating correctly.
It is strange that you've got fluctuate TEER values within your blank. By the way I always have 150 Ωcm2 within my membrane (without cells).
Hi Wafda. I'd agree, the blank reading shouldn't vary massively from day-to-day. Also, 700 is very high for a blank - readings are usually in the region of 120 to 150. You may need a new electrode for the TEER instrument. The electrodes are quite easily damaged, and in the past when we've had issues with odd TEER readings, it has usually been the electrode.
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