Is it necessary to use ethanol to drain off glutaraldehyde?

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Madhukar Baburao Deshmukh

If glutaraldehyde is acting as a spacer to cross-link a primary amino and an enzyme on plat glass then it is not necessary to wash with ethanol.

it necessary to use ethanol to drain off

Tushar Chakraborty

You need to remove glutaraldehyde before proceeding to the enzyme addition step as glutaraldehyde may interfere with the enzymatic activity. For glutaraldehyde removal ethanol is suggested. However, the presence of ethanol will again interfere with your enzyme. So consider removing it by washing with water at least twice.