Is it possible to remake cDNA from RNAs left in the cDNA solution?

By mistake I took concentrated RNA (5ug) for cDNA synthesis(20ul reaction mix) and cDNA synthesis didn't happen. When I ran 2ul of that cDNA (after diluting to 50ul) in a gel, I could see RNA...

09 October 2014 1,282 6 View

Why is DNA not dissolving?

For detecting fragmented DNA from cancer cell line, I isolated DNA with ethanol precipitation and I got a very good DNA pellet. I put the DNA pellet in TE buffer and kept it at 37 degrees...

07 August 2013 3,621 13 View

Disodium EDTA (0.5M) dissolved at ph 7.65. Any help?

I was making 0.5M EDTA and I knew it will dissolve only around ph 8. I was adjusting the ph with 10N NaOH and at ph 7.65 itself whole EDTA dissolved completely. I don't know why it happened like...

07 August 2013 8,001 12 View

Which is the best miRNA target prediction tool available online?

Can anyone please suggest which is the best miRNA target prediction tool that is available online?

01 February 2013 8,457 12 View

How do i prepare mammalian cell lysate for zymography?

I want to do zymography , for that i have seperated my cells and media,i've pelleted my cells and now i want to lyse it for zymography, can anyone please suggest me a good method for that so that...

01 February 2012 5,215 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View