Jinsha Koroth

6 Questions 7 Answers 0 Followers

Questions related from Jinsha Koroth

Is it possible to remake cDNA from RNAs left in the cDNA solution?

By mistake I took concentrated RNA (5ug) for cDNA synthesis(20ul reaction mix) and cDNA synthesis didn't happen. When I ran 2ul of that cDNA (after diluting to 50ul) in a gel, I could see RNA...

10 October 2014 1,361 6 View

Is it necessary to run CEN and CTN every time when you do cell cycle analysis in BC gallios?

Do we have to run CEN and CTN samples and adjust linearity every time before doing cell cycle analysis in BC Gallios system? Or once it is adjusted, the same protocol can we use for cell cycle...

10 October 2014 451 0 View

Disodium EDTA (0.5M) dissolved at ph 7.65. Any help?

I was making 0.5M EDTA and I knew it will dissolve only around ph 8. I was adjusting the ph with 10N NaOH and at ph 7.65 itself whole EDTA dissolved completely. I don't know why it happened like...

08 August 2013 8,053 12 View

Why is DNA not dissolving?

For detecting fragmented DNA from cancer cell line, I isolated DNA with ethanol precipitation and I got a very good DNA pellet. I put the DNA pellet in TE buffer and kept it at 37 degrees...

08 August 2013 3,700 13 View

Which is the best miRNA target prediction tool available online?

Can anyone please suggest which is the best miRNA target prediction tool that is available online?

02 February 2013 8,504 12 View

How do i prepare mammalian cell lysate for zymography?

I want to do zymography , for that i have seperated my cells and media,i've pelleted my cells and now i want to lyse it for zymography, can anyone please suggest me a good method for that so that...

02 February 2012 5,266 1 View