What is the explanation of broad peak in cyclic voltammetric curve?

What is the difference between broad and sharp cyclic volatmmetric curves? and what are the reasons for broad curve? especially when analyzing carbon material?

01 February 2019 9,802 1 View

The llight produced from bioluminescent bacteria, is it able to stimulate photosynthesis in other nearby bacteria and other photosynthetic organisms?

Light produced by bioluminescent microorganisms is composed of photons, are they able to stimulate photosynthesis in other marine organisms? Thsnk you.

03 April 2018 3,318 0 View

What is more accurate to study the effect of pH on bacterial activities, preparing different buffers at different pHs, or adjusting the medium pH?

I am preparing to study the effect of pH on bacterial activities, what is the perfect way for that: 1. Using different buffers and adding the medium components? or 2. Adjusting the medium pH by...

02 March 2018 3,995 2 View

How can I write in my thesis that a Pseudomonas isolate is pyocyanin producer?

Is it enough and acceptable to mention that the observation of fluorescent color is a clue that my strain is pyocyanin producer? Thanks

11 December 2017 8,941 0 View

How can I get the heat of combustion in Energy Efficiency equation for MFC?

In the Energy efficiency equation, how can I get the heat of combustion? Thanks.

10 November 2017 5,310 1 View

What is the "Residual curve" regarding the polynomial fitting?

When I run the polynomial fitting on my data, a curve named "Residual curve"b appear after calculation, what is the meaning of that curve? and is it acceptable to use it instead of the fitting...

10 November 2017 1,342 0 View

How can I calculate number of electrons involved in electrochemical reduction of oxygen?

I am working in Microbial fuel Cell (MFC) and want to calculate number of electrons involved in reduction of oxygen. Thanks

10 November 2017 8,456 0 View

What is the fastest and accurate method for extraction and identification of polysaccharide, pyocyanin, and rhamnolipid from culture of Pseudomonas?

Can you help me getting method (s) for rapid extraction and identification of these three Pseudomonas products? Thank you.

10 November 2017 2,134 0 View

How can I obtain slope of any curve from Origin 6.0 or 8.0 software?

How can I use origin in getting the slope of any curve? Thanks.

10 November 2017 5,738 0 View

About polarization and power curves, is there a method to use potentiostat, without the need for changing different external resistances?

The common method of determination of polarization and power curves of Fuel cell is to change different external resistance and read the corresponding voltage and current. I would like to ask is...

10 November 2017 6,276 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

02 March 2021 3,060 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View