What is the explanation of broad peak in cyclic voltammetric curve?

What is the difference between broad and sharp cyclic volatmmetric curves? and what are the reasons for broad curve? especially when analyzing carbon material?

01 February 2019 9,802 1 View

Is it important to add buffer in the cathode chamber of MFC?

Can I use distilled water as catholyte in MFC? or if I use Potassium ferrocyanide, can I dissolve it in distilled water instead of buffer solution?

11 December 2018 1,374 0 View

The llight produced from bioluminescent bacteria, is it able to stimulate photosynthesis in other nearby bacteria and other photosynthetic organisms?

Light produced by bioluminescent microorganisms is composed of photons, are they able to stimulate photosynthesis in other marine organisms? Thsnk you.

03 April 2018 3,318 0 View

What is more accurate to study the effect of pH on bacterial activities, preparing different buffers at different pHs, or adjusting the medium pH?

I am preparing to study the effect of pH on bacterial activities, what is the perfect way for that: 1. Using different buffers and adding the medium components? or 2. Adjusting the medium pH by...

02 March 2018 3,995 2 View

How can I write in my thesis that a Pseudomonas isolate is pyocyanin producer?

Is it enough and acceptable to mention that the observation of fluorescent color is a clue that my strain is pyocyanin producer? Thanks

11 December 2017 8,941 0 View

How can I get the heat of combustion in Energy Efficiency equation for MFC?

In the Energy efficiency equation, how can I get the heat of combustion? Thanks.

10 November 2017 5,310 1 View

What is the "Residual curve" regarding the polynomial fitting?

When I run the polynomial fitting on my data, a curve named "Residual curve"b appear after calculation, what is the meaning of that curve? and is it acceptable to use it instead of the fitting...

10 November 2017 1,342 0 View

What is the fastest and accurate method for extraction and identification of polysaccharide, pyocyanin, and rhamnolipid from culture of Pseudomonas?

Can you help me getting method (s) for rapid extraction and identification of these three Pseudomonas products? Thank you.

10 November 2017 2,134 0 View

How can I obtain slope of any curve from Origin 6.0 or 8.0 software?

How can I use origin in getting the slope of any curve? Thanks.

10 November 2017 5,738 0 View

About polarization and power curves, is there a method to use potentiostat, without the need for changing different external resistances?

The common method of determination of polarization and power curves of Fuel cell is to change different external resistance and read the corresponding voltage and current. I would like to ask is...

10 November 2017 6,276 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View