Is it high molecular weight or aggregated proteins?

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Swarnab Sengupta

As per my experience the upper bands are not like aggregated proteins. The result is good.

Hud Hud

Swarnab Sengupta Thank you, appreciate your help.

Achim Recktenwald

Aggregate bands are usually a bit fuzzy in appearance. Yours are very sharp and well defined. I agree with Swarnab Sengupta , I do not think these are aggregates.

Andreas Eisenreich

No it is no aggregate. Somewaht more likely is that your antibody is not very specific to your protein of interest.