Is it high molecular weight or aggregated proteins?

More Hud Hud's questions See All

Why I am having a dark area in the upper part of my blot?

Hello, Recently, I'm having this strange dark area in the upper part of my western blot. And it happened many times in the same area exactly!! I think it is a running problem, but I can't...

31 December 2019 9,500 3 View

Why I am having different results in RT-PCR using different cDNA from the same RNA extract?

Hello, I am having a problem with RT-PCR, even though I am making cDNA from the same RNA? Is it possible to use the current data (ATTACHED) to figure out the problem?

31 December 2019 1,417 3 View

What is the cause of this problem in western blot?

This is the upper part of a western blot membrane, and as you can see there are some proteins that did not enter the separating gel. Does this affect the level of protein expression? I mean is...

10 November 2019 1,942 4 View

How to transfect mammalian cells with plasmid?

Is it possible to transfect mammalian cells with plasmid without using a viral vector? and what is the process that enables the plasmid to be connected with the cells' DNA?

10 November 2019 8,939 7 View

Why would the expression of a protein gets up and down with the increasing of a drug concentration?

Is there any explanation of this phenomena?

11 December 2018 5,810 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Achim Recktenwald

Aggregate bands are usually a bit fuzzy in appearance. Yours are very sharp and well defined. I agree with Swarnab Sengupta , I do not think these are aggregates.

Andreas Eisenreich

No it is no aggregate. Somewaht more likely is that your antibody is not very specific to your protein of interest.