Why I am having a dark area in the upper part of my blot?

Hello, Recently, I'm having this strange dark area in the upper part of my western blot. And it happened many times in the same area exactly!! I think it is a running problem, but I can't...

31 December 2019 9,500 3 View

Why I am having different results in RT-PCR using different cDNA from the same RNA extract?

Hello, I am having a problem with RT-PCR, even though I am making cDNA from the same RNA? Is it possible to use the current data (ATTACHED) to figure out the problem?

31 December 2019 1,417 3 View

Is it high molecular weight or aggregated proteins?

I am still confused about the upper bands in most of my western blots, is it aggregated protein or high molecular weight?

31 December 2019 546 4 View

What is the cause of this problem in western blot?

This is the upper part of a western blot membrane, and as you can see there are some proteins that did not enter the separating gel. Does this affect the level of protein expression? I mean is...

10 November 2019 1,942 4 View

How to transfect mammalian cells with plasmid?

Is it possible to transfect mammalian cells with plasmid without using a viral vector? and what is the process that enables the plasmid to be connected with the cells' DNA?

10 November 2019 8,939 7 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

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19 February 2021 8,647 3 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

How can I add the metal oxide NP's to the cell lines? I have to make some drug and NP conjuhgates. How much concentration to use? Need some help?

Hi everyone. I am working on metal oxides. I need to make some conjugates of drugs and NP. But I am unsure of what concentrations to make. I have read some articles, but they haven't mentioned...

10 February 2021 3,372 2 View