12 December 2018 2 6K Report

Is there any explanation of this phenomena?

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Why I am having a dark area in the upper part of my blot?

Hello, Recently, I'm having this strange dark area in the upper part of my western blot. And it happened many times in the same area exactly!! I think it is a running problem, but I can't...

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Why I am having different results in RT-PCR using different cDNA from the same RNA extract?

Hello, I am having a problem with RT-PCR, even though I am making cDNA from the same RNA? Is it possible to use the current data (ATTACHED) to figure out the problem?

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Is it high molecular weight or aggregated proteins?

I am still confused about the upper bands in most of my western blots, is it aggregated protein or high molecular weight?

31 December 2019 695 4 View

What is the cause of this problem in western blot?

This is the upper part of a western blot membrane, and as you can see there are some proteins that did not enter the separating gel. Does this affect the level of protein expression? I mean is...

10 November 2019 2,049 4 View

How to transfect mammalian cells with plasmid?

Is it possible to transfect mammalian cells with plasmid without using a viral vector? and what is the process that enables the plasmid to be connected with the cells' DNA?

10 November 2019 9,101 7 View

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