Hi Dhananjay,

although LiCl2 precipitation is less efficient compared to the classic ethanol procedure, you can use those samples for RNA sequencing.

this page could help you: https://www.thermofisher.com/fr/en/home/references/ambion-tech-support/rna-isolation/general-articles/the-use-of-licl-precipitation-for-rna-purification.html.

fred

Thanks Frederic Lepretre, I have seen this Thermo technical bulletin which shows even 5ng/μl concentration of 100 nucleotides is fine for this method. I was willing to use it because of its property to precipitate exclusively RNA and not DNA.

My concern was the size of RNA as its preferentially precipitate bigger sized RNA only and in case the presence of Cl ions which might affect sequencing.

Ok I see,

Cl ions won't affect your sequencing since volume of sample in main reactions is low, buffers used will erase such interference. lot of LiCl2 prepared samples have been sequenced by NGS technologies without problem. take a look at punned, you'll have lot of papers.

all the best

fred