Dhananjay K. Pandey

5 Questions 8 Answers 0 Followers

Questions related from Dhananjay K. Pandey

Is it fine to do RNA sequencing using RNA samples precipitated by LiCl?

Generally, most of kit or protocol suggested the ethanol/isopropanol precipitation of RNA. I am curious about any side effect of using the LiCl method of precipitation especially if the goal is to...

01 January 2020 3,598 3 View

How to calculate trans-gene copy number using real time PCR ?

How much it is correlated with southern blot on the basis of accuracy.

10 October 2016 7,658 2 View

Is it possible to stain plant samples stored at -80 degree with Phalloidin fluorescent dye or only fresh tissue is required for the same??

My sample is fiber tissue and stored at -80 degree. Now I want to stain it with Phalloidin fluorescent dye. Kindly suggest if anyone has experience of such kind.

10 October 2016 9,486 2 View

How to measure folic acid/Folate content in the bacterial culture using spectrophotometer?

Want to measure folic acid produced by bacteria, Is there any specific wavelength fixed for folic acid detection.

02 February 2016 3,275 3 View

How to design specific primer for micro RNA expression analysis using realtime PCR?

As microRNA are very small in size (approx 25 base long)

03 March 2014 7,461 5 View