Hi,

You don't have to do design microRNA primers. Companies that provide these reagents had already designed them for you, and sell them ready to use. Please check the website of companies that sell these reagents, such as Exiqon, Qiagen, Dharmacon and others.

Good luck!

Cheers,

Paulo

Hi,

You don't have to do design microRNA primers. Companies that provide these reagents had already designed them for you, and sell them ready to use. Please check the website of companies that sell these reagents, such as Exiqon, Qiagen, Dharmacon and others.

Good luck!

Cheers,

Paulo

I would also recommend using existing systems that have been well-tested and published. I use the Applied Biosystems TaqMan system and sometimes Exiqon. However, some feel they can save money by designing and ordering their own primers. To this end, see previous discussion, including: https://www.researchgate.net/post/Where_to_find_the_forward_primer_sequences_for_various_miRNAs_when_analyzing_miRNA_expression_using_RT-PCR

There are also numerous papers, e.g.:

https://www.researchgate.net/publication/235403016

Article A Versatile Method to Design Stem-Loop Primer-Based Quantita...

Thanks Dr. Bettencourt and Dr. Witwer for your kind suggestions. Now, I will look forward in this direction for readymade primers.

Hi everybody

I agree with Paulo, however sometimes you need to use your imagination for example with miRNAs which are not already well characterized. In this case, try to apply the protocol described in this paper. Hope it helps

http://www.biomedcentral.com/1471-2105/15/29

Best

Paco