I did that in the past. It kind of works ( I used a GFP driven by a Wnt-reporter and different treatments), you just need high expression levels, because the signal to noise ratio is way beyond e.g. luciferase. I would give it a try.

It also worked for me. I performed flow cytometry to estimate the transfection eficiency and then proceeded with the best condition. I performed western blot and I could see the protein with an anti-GFP antibody. 

yes it works . you can try .

I will be careful with RIPA as you want to see a fluo signal from a protein... (i never try this, but i compared signals by simple counting/measuring intensity on microscopic pics).

I would use the FACS also for the screen as you just collect the cells in PBS and measure fluorescence. It is much easier and safer than the lysis

Thank you all! I will take your advice into consideration.

Hi Cristina,

I am doing the same type of experiment to see ro-GFP signal by fluorimeter in lysed heart tissue.Could you please let me know did you use SDS in your RIPA buffer? or what are the best conditions to read GFP signal by fluorimeter?

Thanks

Hi.

Using of SDS in RIPA buffer to detect ro-GFP will tamper the fluorimetric signals. SDS directly effects the ro-GFP (cysteines moieties) due to its strong negative charges.

In the case of GFP, RIPA buffer without SDS and no or reduced quantities of sodium deoxycholate can do better job to detect GFP signals intact. If SDS is added, boiling of samples should be avoided to preserve the GFP signals. Thread: https://www.researchgate.net/post/In-gel_GFP_fluorescence_with_SDS-PAGE_protocol.

All the best.

hi Cristina. i was plaining to do the exact assessment. May you provide updates? did you tried and worked? thank you