13 Questions 26 Answers 0 Followers
Questions related from Alexander Bontempo
Hi, i'm looking to expose my cells culture to ultrasounds. I need a way to expose the cells to 0.5MHz, 1Khz PRF with an intensity of 0.35-0.5MPa. Any suggestion?
13 July 2023 3,983 1 View
hi, i did a qPCR to quantify HIV integration using TZM.bl. i did a PCR targeting gag gene in genomic DNA. i can't find an explanation why TZM.bl wild type came out highly positive. I'm missing...
16 March 2023 5,518 2 View
Hi i'm using microamp plates on a quantstudio 3. the plate basically just touches on the left and right side on two pieces of plastic while the rest of the plate is flying. I call ab and they say...
28 February 2023 7,745 0 View
hi, i tried to transfect Tzm.bl to stably express a protein. When i used G418 as selection marker the non-transfected cells didn't die. Anyone knows if for establishing the tz.bl from Hela neo...
15 February 2023 8,703 0 View
Hi, i was planing to use transfer plasmid and a HIV backbone plasmid containing gag to assessed integrase activity. To do that im missing an information. When i transfect a cell with both plasmid...
09 February 2023 6,007 1 View
Hi, i'm desesperate. i'm trying to produce lentivirus usinf BEI resources kit NR-52948. 24 hours after transfection i detect a massive GFP expression in my 293T cells. After 72 hours i filtered...
31 December 2020 10,239 6 View
Hi, i tried twice to introduce some mutation and i end up with parental all the time. I prolonged the dpni digestion overnight but also that didn't help. Is possible dpni is not working...
18 December 2020 9,163 0 View
hi guys, i need to predict, given a structural protein back bone what are the position that can lead to a disulfide bound. i need it cause my purpose is to insert a disulfide bridge in a protein...
15 April 2020 7,953 3 View
I performed the Tm assessment of two proteins trough nanoDSF. The two graphs have different width and amplitude. Has this a meaning? How is different width interpreted?
07 March 2020 8,746 2 View
The different APC cells present and process the same epitopes? all the APC present the same MHC:peptide complexes or there are differences between the different APC cell types? i mean a DC...
01 April 2019 6,027 5 View
Hi i have a quiz for you guys. i don't know how to solve this trick. attached you ll find flow chart with my issue in dettails. Briefly, i tried to purify a plasmid trough maxiprep (two different...
07 November 2018 1,520 5 View
Hi, this is my first time i try protein expression so i need a really complete protocol to perform CHO-K1 transfection using PEI scalable up to one liter culture. I need a protein expression for...
08 May 2018 2,835 2 View
I saw some gromatography graphs and i can't understand why gp140 trimers who should weight 412kDa shows the peak between ferritin (440kDa) and thyroglobulin (669kDa) references. Thank you.
22 January 2018 9,920 2 View
