Alexander Bontempo

10 Questions 24 Answers 0 Followers

Questions related from Alexander Bontempo

Nobody knows about any ultrasound emitter for invitro experiments?

Hi, i'm looking to expose my cells culture to ultrasounds. I need a way to expose the cells to 0.5MHz, 1Khz PRF with an intensity of 0.35-0.5MPa. Any suggestion?

13 July 2023 3,916 1 View

0.1ml 96 on quantstudio 3 is it notmal they move and not touch the block?

Hi i'm using microamp plates on a quantstudio 3. the plate basically just touches on the left and right side on two pieces of plastic while the rest of the plate is flying. I call ab and they say...

28 February 2023 7,677 0 View

Are tzm.bl neomycin resistant?

hi, i tried to transfect Tzm.bl to stably express a protein. When i used G418 as selection marker the non-transfected cells didn't die. Anyone knows if for establishing the tz.bl from Hela neo...

15 February 2023 8,654 0 View

Transfer plasmid us integrated in cell producers?

Hi, i was planing to use transfer plasmid and a HIV backbone plasmid containing gag to assessed integrase activity. To do that im missing an information. When i transfect a cell with both plasmid...

09 February 2023 5,956 1 View

I really need help with cov-2 pseudotyped lentivirus production. Support?

Hi, i'm desesperate. i'm trying to produce lentivirus usinf BEI resources kit NR-52948. 24 hours after transfection i detect a massive GFP expression in my 293T cells. After 72 hours i filtered...

31 December 2020 10,021 6 View

Quickchange mutagenesis troubleshooting?

Hi, i tried twice to introduce some mutation and i end up with parental all the time. I prolonged the dpni digestion overnight but also that didn't help. Is possible dpni is not working...

18 December 2020 9,100 0 View

Has the width of a nanoDSF graph some meaning?

I performed the Tm assessment of two proteins trough nanoDSF. The two graphs have different width and amplitude. Has this a meaning? How is different width interpreted?

07 March 2020 8,693 2 View

Why my mini prep is working but no my maxi considering same bacterial culture?

Hi i have a quiz for you guys. i don't know how to solve this trick. attached you ll find flow chart with my issue in dettails. Briefly, i tried to purify a plasmid trough maxiprep (two different...

07 November 2018 1,462 5 View

Nobody has a detailed protocol for CHO transfection with PEI?

Hi, this is my first time i try protein expression so i need a really complete protocol to perform CHO-K1 transfection using PEI scalable up to one liter culture. I need a protein expression for...

08 May 2018 2,777 2 View

Why hiv gp140 trimers in chromatography shown a peak between ferritin and thyroglobulin references?

I saw some gromatography graphs and i can't understand why gp140 trimers who should weight 412kDa shows the peak between ferritin (440kDa) and thyroglobulin (669kDa) references. Thank you.

22 January 2018 9,840 2 View