15 Questions 31 Answers 0 Followers
Questions related from Alexander Bontempo
Hi, i'm looking to expose my cells culture to ultrasounds. I need a way to expose the cells to 0.5MHz, 1Khz PRF with an intensity of 0.35-0.5MPa. Any suggestion?
13 July 2023 3,983 1 View
hi, i did a qPCR to quantify HIV integration using TZM.bl. i did a PCR targeting gag gene in genomic DNA. i can't find an explanation why TZM.bl wild type came out highly positive. I'm missing...
16 March 2023 5,520 2 View
Hi i'm using microamp plates on a quantstudio 3. the plate basically just touches on the left and right side on two pieces of plastic while the rest of the plate is flying. I call ab and they say...
28 February 2023 7,755 0 View
hi, i tried to transfect Tzm.bl to stably express a protein. When i used G418 as selection marker the non-transfected cells didn't die. Anyone knows if for establishing the tz.bl from Hela neo...
15 February 2023 8,705 0 View
Hi, i was planing to use transfer plasmid and a HIV backbone plasmid containing gag to assessed integrase activity. To do that im missing an information. When i transfect a cell with both plasmid...
09 February 2023 6,010 1 View
hi, i'm assessing the effect of dead bacteria on viability of fibroblast. Both control bacteria alone and cells alone don't show any change in medium color but the co-culture become yellow. The...
16 August 2021 759 7 View
Hi, i'm desesperate. i'm trying to produce lentivirus usinf BEI resources kit NR-52948. 24 hours after transfection i detect a massive GFP expression in my 293T cells. After 72 hours i filtered...
31 December 2020 10,247 6 View
Hi, i tried twice to introduce some mutation and i end up with parental all the time. I prolonged the dpni digestion overnight but also that didn't help. Is possible dpni is not working...
18 December 2020 9,165 0 View
hi guys, i need to predict, given a structural protein back bone what are the position that can lead to a disulfide bound. i need it cause my purpose is to insert a disulfide bridge in a protein...
15 April 2020 7,955 3 View
I performed the Tm assessment of two proteins trough nanoDSF. The two graphs have different width and amplitude. Has this a meaning? How is different width interpreted?
07 March 2020 8,748 2 View
Hi. Hiv is recognize to be indirectilly oncogenic afecting immune system and altering cell cicle but not for integration. So why there are major concern about insertional risk on hiv-based vectors...
04 June 2019 4,117 0 View
The different APC cells present and process the same epitopes? all the APC present the same MHC:peptide complexes or there are differences between the different APC cell types? i mean a DC...
01 April 2019 6,028 5 View
Hi i have a quiz for you guys. i don't know how to solve this trick. attached you ll find flow chart with my issue in dettails. Briefly, i tried to purify a plasmid trough maxiprep (two different...
07 November 2018 1,520 5 View
Hi, this is my first time i try protein expression so i need a really complete protocol to perform CHO-K1 transfection using PEI scalable up to one liter culture. I need a protein expression for...
08 May 2018 2,838 2 View
I saw some gromatography graphs and i can't understand why gp140 trimers who should weight 412kDa shows the peak between ferritin (440kDa) and thyroglobulin (669kDa) references. Thank you.
22 January 2018 9,924 2 View
