Is it correct to take the initial DPPH solution used as the A0 value in the DPPH radical scavenging test?
For example; When 1 ml of sample solution of known concentration is added to 3 ml of DPPH solution, the total volume will change and therefore the initial DPHH concentration will also change. Is it necessary to get results by adding the amount of solvent (ethanol or methanol) to the DPPH solution in the amount of sample volume added while reading the A0 value?
To use DPPH as blank you need to take the same volume as you are using with your sample. For that, you have to add the solvent instead of your sample in the blank. The assay volume should be equal in the case of the sample and the blank.
I can recommend that you optimize your method so that you always add one volume of sample, which you will dilute separately before adding to the reaction mixture.
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Extraction