Books on Epoxies and Plastics?

Possibly because I work a lot with ocean deployed devices, I have a lot of encounters with potting and marinizing using epoxies and silicones. I usually am working on intuition and the word of...

25 October 2020 9,094 3 View

Is there a criteria for disqualifying animals if they don't interact enough? How much (in seconds) is too little of an interaction?

19 May 2016 6,428 3 View

Is there a criteria for disqualifying animals if they don't interact enough? How much (in seconds) is too little of an interaction?

04 May 2016 8,396 4 View

Does anyone have a flow cytometry protocol using DCFDA?

18 February 2015 9,040 5 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

How to accurately measure cytokines levels for my university research proposal?

Hello, i am writing a theoretical research proposal for one of my university assignments regarding Covid-19. The brief is pretty relaxed and i have chosen to conduct a study on how the...

11 February 2021 908 5 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

What M1 and M2 markers can I use for flow cytometry ?

Hi, I'm a student and I'm doing an internship. I need to asses the M1 and M2 populations of a culture using flow cytometry. From my researches, I know that this is subject to some controversy, as...

26 January 2021 299 3 View

Does re-stimulation of T cells with PMA and Ionomycine before staining for intracellular cytokines for flow cytometry affect reliability of the assay?

I understand that to be able to measure intracellular cytokines there is a need to restimulate with PMA and Ionomycin but I don't understand how the results will be reliable after that. PMA and...

18 January 2021 7,240 7 View

Cytokine levels in tears sample?

Hi dear, Do you have any idea of a commercial lab who measure the cytokines levels in tears by multiplex or flow cytometry? Thank you

18 January 2021 5,928 3 View

Did I dilute my supernatant too much so cytokines will be below the limit of detection on a Luminex assay?

I am performing an experiment analyzing the cytokine production of macrophages in response to viral infection. I recently harvested and cultured peritoneal macrophages from mice. I plated these...

17 January 2021 5,516 3 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

How long do mouse CD4+ T-cells remain activated after removal of CD3/28?

Hello, I would like to analyse naive CD4+ T-cell activation and intracellular processes with a machine that does not allow CD3-coated plates. I thought of activating my cells with plate-bound...

08 December 2020 3,536 1 View