Dear colleagues,
I will measure serum Ang 1, Ang 2 and VCAM-1 levels by ELISA in patients who were undergone hematopoietic stem cell transplantation. I should find the most suitable dilutions to be able to measure the cytokines' levels within the limits of the standards in the ELISA kit. I will try different dilutions, but I should use the wells of the kit as few as possible for the dilution optimization. Does anyone have any experience or recommendations which dilutions would be suitable while measuring serum Ang 1, Ang 2 and VCAM-1 levels?
Standard Curve Range for Ang 1 is 150-2400 pg/mL.
Standard Curve Range for Ang 2 is 40-640 ng/mL.
Standard Curve Range for VCAM-1 is 1.56-100 ng/mL.
Thank you very much for your help.
Hello. Our lab has found that a good dilution factor for VCAM-1 within both postmortem and antemortem human serum is 50X. I hope this helps.
https://jvsvs.org/article/S2666-3503(24)00017-8/fulltext
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