I want to obviate the centrifuge steps for DNA Isolation.
Your question not clear enough to help you. You need to state reason of skipping centrifugation. The Microbes can only be concentrated thro that means to obtain enough cells for DNA extraction.
I inserted my D-product artificial-miRNA into pDONR207 DH5alpha, colony confirmed with PrimerA , PrimerB also with attB1, and attB2 primers. similarly, the plasmid was confirmed with the same...
14 September 2023 6,144 3 View
I want to synthesized one Probe from one publish article, but its showing like "T" in between the sequence? Can anyone knows what is the meaning for the same? [6FAM]...
09 July 2020 9,220 0 View
I am developing HIV Viral Load Detection Assay, in that my Quantitative Standards are in Copies/mL, but I want to convert it to International Unit (IU)/mL as WHO recommendation to give results in...
27 September 2019 6,314 7 View
I am using primer & Probe concentration 10 uM and using 0.5 uL in 25 uL reaction PCR setup. I have used so many different water but each water coming positive. Its might be primer dimer but...
05 February 2019 358 10 View
I have developed RT PCR mastermix , now its required to add stabilizer in it? if yes which stabilizer we can use at which concentration.
02 December 2018 1,794 9 View
Hello Friends, I am looking for a open source software, which can allow me to monitor the parameters of a wireless network. 1. Serving Cell RSRP, RSRQ & SINR. 2. Neighbor Cells RSRP. 3....
31 August 2018 6,625 6 View
I want to amplify DNA with Internal Control
21 August 2018 566 3 View
I want to know the artificial gene or strain which can be use as a DNA/RNA extraction control, and will lead you to RT PCR testing for any viral DNA or RNA.
10 April 2018 8,537 4 View
I want to stabilize the RT PCR Master Mix at Room Temperature . Please suggest if any one having same experience or some stabilizer i can try.
06 October 2017 9,488 3 View
I have used this technique for the 2-D heat equation but am not sure how to apply it to a system of equations (i.e. a vector PDE). Do the errors for the dependent variables have the same Fourier...
22 April 2016 1,530 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View