I am working on Alzheimer's disease using a cell line model. I want to know if HT22 cell line is appropriate to measure Beta secretase activity, amyloid aggregation, and expression of amyloid precursor protein including other secretases.
I am researching parents who have autistic children and what are the challenges they are facing in rural communities in Nigeria.
16 June 2023 644 6 View
Explanation on the implications of new technologies as regards the society and the effect it has placed in the past, effects on the present world(generation), and future effects.
05 February 2023 9,495 3 View
Please, I was wondering if anyone has any idea on the duration for validating a gene knockout using CRISPR or RNAi? I have a choice to either use RNAi or CRISPR but I want to know which one will...
13 July 2022 6,893 0 View
Question 1 (25 Marks) ‘The overall goal of the People Plan in 2018 remains much as it was in 2016: to ensure that the department has skilled, talented, diverse and high-performing people, who are...
28 March 2022 4,314 1 View
Hi all, Please I will like to know the hypervariable region of bacteria gene that will reveal abundant taxonomic composition from metagenomic DNA.
20 February 2020 806 1 View
The sequence DNA will be extracted directly from water and sediment.
07 November 2019 3,878 7 View
I am looking to quantify the surface roughness parameters of some of rock grain samples I recently analysed with an optical profilometer. The information that was obtained from the equipment was...
02 September 2018 6,630 3 View
should the steel/alloy be flat or round in shape, flat plate or pipe-like and also what thickness, length and width should i use.
10 October 2016 584 8 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View