The sequence DNA will be extracted directly from water and sediment.
Hi Tosin,
The selection of tool for metagenomics depends upon the computational resources available to you. There are plethora of tools which can you use :
Server based:
MG-RAST:http://www.mg-rast.org/
Gaia: https://metagenomics.sequentiabiotech.com/
SilvaNGS: https://www.arb-silva.de/
Galaxy platform :https://usegalaxy.org/
Kaiju: http://kaiju.binf.ku.dk/server
Standalone:
Mothur
QIIME
I hope this helps.
Best Wishes
Pankaj
If you want to do everything, Quality check, assembly, annotations, Diversity etc QIIME is the best. For only taxonomic diversity MOTHUR is enough. Both the software run on LINUX platform.
Qiime (http://qiime.org/) or MOTHUR .
Thank you so much Pankaj Kumar Singh . I would check them out.
Aww thank you Sahar Abdelrazek
Thank you V.J Rejish Kumar
Squuezemeta pipeline (https://github.com/jtamames/SqueezeMeta)
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