The sequence DNA will be extracted directly from water and sediment.
Hi Tosin,
The selection of tool for metagenomics depends upon the computational resources available to you. There are plethora of tools which can you use :
Server based:
MG-RAST:http://www.mg-rast.org/
Gaia: https://metagenomics.sequentiabiotech.com/
SilvaNGS: https://www.arb-silva.de/
Galaxy platform :https://usegalaxy.org/
Kaiju: http://kaiju.binf.ku.dk/server
Standalone:
Mothur
QIIME
I hope this helps.
Best Wishes
Pankaj
If you want to do everything, Quality check, assembly, annotations, Diversity etc QIIME is the best. For only taxonomic diversity MOTHUR is enough. Both the software run on LINUX platform.
Qiime (http://qiime.org/) or MOTHUR .
Thank you so much Pankaj Kumar Singh . I would check them out.
Aww thank you Sahar Abdelrazek
Thank you V.J Rejish Kumar
Squuezemeta pipeline (https://github.com/jtamames/SqueezeMeta)
I am looking to quantify the surface roughness parameters of some of rock grain samples I recently analysed with an optical profilometer. The information that was obtained from the equipment was...
02 September 2018 6,462 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...
01 March 2021 2,512 1 View
Source to get Impact factor of journals
01 March 2021 6,654 2 View
There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.
26 February 2021 5,851 1 View
There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...
26 February 2021 3,404 10 View
For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...
23 February 2021 1,346 13 View
Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...
18 February 2021 371 4 View
We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...
16 February 2021 678 3 View
I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...
16 February 2021 5,101 2 View
There are 6 television programmes of different TV genres and their mean and mean ranks are put for each of them. But the reliability value of all the programmes together is 0.498 . So what exactly...
15 February 2021 7,686 4 View