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Dear all, I am trying to isolate and culture bovine endometrial stromal cells (stromal fibroblast cells) by using collagenase type II for digestion (conc. 2mg/ml). I tried tow times but the result...
03 April 2016 9,874 11 View
Dear friends and fellows, It will be great to know your experiences working with extracellular/circulating or exosomal miRNAs. We are facing different problems starting from sample isolation to...
01 February 2016 9,270 2 View
Dear all, I am currently using Qiagen miRNA profiler plates (catalogue number: MIBT-659ZF-24) to identify deregulated miRNAs in cattle under certain physiological condition. I have ordered the...
11 December 2015 3,592 5 View
Dear all, I am working with granulosa cells and I would like to know- 1. Is it possible to develop a cell line using granulosa cells? 2. If possible how many passage it require?
10 November 2015 1,503 4 View
Dear all, I am working with bovine granulosa cells. Recently, I have moved to a new lab and I lost my previous protocol to prepare RBC lysis buffer which is suitable for granulosa cells collected...
01 February 2015 5,063 10 View
I am trying to to interpret miRNA expression in certain sample. I have only raw ct value. It would be very nice to include concentration in relation to ct value.. has anyone done it??? help and...
02 March 2014 3,620 3 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
