I know protein structure is very susceptible to degradation as a result of multiple freeze-thaw cycles. But is it a concern for a sugar like LPS?
Asking because I have 50 uL aliquots of LPS and I only use 1 uL at a time. Wondering if it's worth it to aliquot it further and/or if I should be recording freeze/thaw cycles for each aliquot.
Thank you Ebrahim for your response. That is an interesting article, but I am more interested in whether freeze-thawing a solution of LPS itself (not bacteria) is detrimental to its function. I'm imagining the answer is "no" but wanted to get others' input. Thanks again!
I have heard mixed reviews on freezing/thawing LPS. I know several people who say that it is very unstable and shouldn't be frozen/thawed more than once. I have also heard others say it is relatively stable. To be safe, I would aliquot it further and only use it once and not refreeze it.
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