I'm trying to do double IHC-p/IF on mouse lung tissue. One Ag does not show up. Why is this?

I am trying to do double IHC-p/IF on mouse lung tissue.  For the 1st antigen, Fas, I have a polyclonal rabbit-anti-mouse Fas primary antibody and a goat-anti-rabbit AF488 secondary antibody. For...

02 February 2016 8,043 7 View