Kelly Hanson

5 Questions 9 Answers 0 Followers

Questions related from Kelly Hanson

How to compare ratios of proteins with different exposure times? (Western blot)

Hello everyone, I am blotting for caspase 3. I have an antibody that binds to both cleaved and full length caspase 3. However, when I expose the blots, there is much more full length caspase 3...

19 July 2017 2,371 4 View

Is freeze-thawing a concern for LPS (lipopolysaccharide)?

I know protein structure is very susceptible to degradation as a result of multiple freeze-thaw cycles. But is it a concern for a sugar like LPS? Asking because I have 50 uL aliquots of LPS and I...

28 March 2017 3,330 4 View

In Westerns, what would cause 1 positive band in the ladder lane?

Hello everyone, I am getting a strong positive band from my ladder lanes. However, it doesn't even match up in size with any of the stained standards! It is showing up at maybe 60 kDa, between...

21 June 2016 7,330 7 View

I'm trying to do double IHC-p/IF on mouse lung tissue. One Ag does not show up. Why is this?

I am trying to do double IHC-p/IF on mouse lung tissue. For the 1st antigen, Fas, I have a polyclonal rabbit-anti-mouse Fas primary antibody and a goat-anti-rabbit AF488 secondary antibody. For...

02 February 2016 8,109 7 View

What are the phenotypic differences between primary mouse type II alveolar epithelial cells and the MLE15 cell line?

I am trying to build a triculture model of the lung with type II alveolar epithelial cells (AEC's), lung fibroblasts, and conditioned media from lung macrophages. My goal is to keep the AEC...

18 August 2015 3,985 2 View