Sidney T Sithole

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Questions related from Sidney T Sithole

What is the optimal G418 Selection Strategy for Stable Transfection in HUT78 (CD4+ T Cell Line)?

I want to stably transfect HUT78 cells (a CD4+ T cell line) with a plasmid carrying a neomycin resistance gene and will begin selection with G418 (protocol recommends 400ug/ml). For those with...

12 June 2025 9,422 3 View

Is qPCR validation of RNA-seq data required just to satisfy reviewers, or is the field moving beyond that?

RNA-seq already gives us quantitative expression data, so do we really need qPCR validation—or should we be focusing on protein-level confirmation instead? And if RNA-seq and qPCR don’t agree,...

01 January 1970 3,913 4 View