Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

What are the different subclasses in intermediate seeds?

I am currently working on an endemic tree species, that produces mature seeds (moisture content 51%) which shows both vivipary (30% of total harvested seeds from a tree) and dormancy (~40%) of...

20 January 2021 2,670 3 View

Do you can put again my publications online?

Dear All, Please I has accidentaly removed 22 publications from RG my profile in the mail that you send me today, please put again those publicatios in my profile. Best regards,

13 December 2020 9,981 4 View

Adhesion energy (MOF-water) >> cohesion energy (MOFs) results higher uptake- how can I prove it thermodynamically?

In a surface energy analysis, we have found a very interesting result- the cohesive energy of aluminum fumarate is lower than the adhesive energy of aluminum fumarate-water pair. Also, the uptake...

05 November 2020 4,292 2 View

How can one automatically extract drainage basin values from a DEM following Strahler stream ordering approach?

Analysing hydrologically corrected DEM avails an opportunity to calculate morphometric indices such as drainage basin density(DD) (which takes manual approach). Can not DD and others be extracted...

07 October 2020 3,893 3 View

How to hydroxylate the surface of bismuth telluride nanoparticles?

Would it be possible to add -OH functional groups to Bi2Te3 nanoparticles?

07 October 2020 6,560 3 View

What is the chemical stability of bismuth telluride either in bulk or nanoparticle form (e.g., nanowires, platelets, etc)?

I am interested in post-synthethic methods of this material, and would like to know the chemical stability of Bi3Te2 (particularly nanotubes) in water, acid o alkaline media. Any literature...

03 October 2020 2,743 3 View

Does reducing the brain neuronal network as an inevitable aging event serve to consolidate memory tracking?

07 September 2020 6,188 2 View

What are all the criteria for the occurrence of Lunar Eclipse?

I have read the maximum limit of Lunar Eclipse is 12.2 degrees of arc (i.e. the difference in the longitude between Moon and any one node of the Moon). However on on June 5th 2020, a partial Lunar...

27 August 2020 986 5 View

Are there differences between Rietveld refinement process for powder sample and thin films sample?

In general thin films exhibit a preferred direction, and other features like contribution of substrate in XRD pattern....ext. So I would like to ask : if we use the Rietveld refinement with XRD...

23 July 2020 9,725 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View