Can someone tell me if Dulbecco′s Modified Eagle′s Medium - high glucose With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered is suitable for CHO-K1 cell growth and in vitro toxicology research? Will the cells grow well in such a medium? Can the components of the medium affect the toxicity of the metals to the cells?
Thank you in advance for all answers.
Google search appears t,o render appropriate answers. I personally haven't grown CHO cells for over 40 yrs. See https://www.researchgate.net/post/Can-I-culture-CHO-cells-with-DMEM-FBS for example. CHO require proline and NaPyruvate. You need to assess if your experiments will tolerate 10% or greater FBS. If not, using a more CHO-specific designed CM might give you greater latitude. Yes, components of any solution can impact cell-based endpoints, e.g., metal chelation, binding to serum components, etc. It'll be your responsibility to control for those factors as you conduct your expts. For example, lot-to-lot variability of FBS can play a role if not mitigated. Good luck!
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