What are the main problems associated with LDPE recycling or valorization compared to other polymers such as HDPE and PET?

In most developing countries like Nigeria, low to medium-income earners consume packaged water coming from sachet water. Sachet water is made from LDPE. I am looking at each packaging holistically...

13 October 2020 5,044 3 View

What is the heat energy in an isothermal process?

I was conducting furnace testing (Carbolite ELF 11/6b) on a carbon epoxy composite in order to find fibre volume fraction. I was recently working on the thermodynamics of the process. Obviously in...

27 June 2020 679 1 View

How stable is Alpha-1 Antitrypsin in frozen samples?

Recently I observed that my samples containing human AAT loose its concentration when stored at -20oC. Does anybody have an experience in safe conditions for storing AAT samples- how long and in...

19 July 2018 7,136 4 View

Do anybody know, good (free-download) program for performing attractive visualisation of structures derived from Gaussian calculations?

Do anybody know, good (free-download) program for performing attractive visualisation of structures derived from Gaussian calculations? Options from GaussView 5.08 are rather primitive. Thanks...

13 October 2017 3,346 16 View

Problem with white colour of wastewater

I have the following problem. Municipal wastewater is treated on biological beds. The beds are polypropylene open mesh baskets which offer high area for growth of microorganisms. The beds are...

25 May 2017 1,538 4 View

Did anyone try to fix the cells with glutaraldehyde before using ThiolTracker?

08 February 2016 7,451 3 View

Why is in vivo treatment increasing auto-fluorescence?

17 September 2014 556 4 View

Is it possible to jitter horizontally individual data points in SPSS?

I have many overlapping data points and I wonder if it is possible to jitter them only in one direction, on the x axis where I have categorical variables. I was trying various commands in the...

18 August 2013 2,090 4 View

Heparin neutralization in heparanised whole blood samples from patients?

What is the best method to neutralize non-fractionized heparin and LMW heparin in patient blood for coagulation analysis (thromboelastography). Is heparinase I enzyme recommended? Which dose...

04 August 2013 7,920 18 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View