I want extraction RNA from tissue in which ocurre blood clots due to DIC. Which way will be the best to removing blood clots from tissues? Dipping a tissue in sterile PBS? Or however perfusion of tissue with sterile PBS or RNAlater?
I am investigating chemical immunotoxic potential in mice dermally exposed to a chemical for 10 consecutive days. Mice were then immunized with SRBC by tail injections of the antigen. Three days...
10 July 2023 8,884 0 View
Can someone tell me if Dulbecco′s Modified Eagle′s Medium - high glucose With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered is suitable for...
10 October 2022 6,540 1 View
I have run ordered probit model for a latent factor using gsem function in Stata 16. This is because I have Likert scale (1-5) answers to my observable variables. The problem is I cannot obtain...
13 May 2021 9,365 5 View
We design a lot of hall buildings with just unbraced cantilever concrete columns with light steel construction of the roof on top of it. Sometimes there must be a fire resistant wall to isolate...
01 May 2021 9,078 3 View
I am looking for information regarding pH influence for the peaks shift in the low aliphatic region of the protein NMR spectrum. In my research, I've performed fragment-based screening of the...
02 March 2021 9,999 2 View
In most developing countries like Nigeria, low to medium-income earners consume packaged water coming from sachet water. Sachet water is made from LDPE. I am looking at each packaging holistically...
13 October 2020 5,253 3 View
I carried out multiple TGA tests on a carbon-epoxy composite. It was performed in nitrogen, in the temp range 20-800C at heating rates of 5, 10 and 20C/min. For 5C/min, above 600C, a slow,...
17 August 2020 4,863 3 View
I was conducting furnace testing (Carbolite ELF 11/6b) on a carbon epoxy composite in order to find fibre volume fraction. I was recently working on the thermodynamics of the process. Obviously in...
27 June 2020 820 1 View
Which species of fungi of the genus Fusarium are dominant in grain in your country?
14 July 2019 9,957 11 View
In my studies I used image analysis, thermal analysis and PCR. What is the best and most reliable method?
09 July 2019 2,739 14 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
This is a slice of intestinal tissue from the Chinese mitten crab (intestine in the upper right corner). I injected WSSV into this horseshoe crab. Now I want to know what these red spots next to...
18 July 2024 5,602 0 View
The burden of obesity is enormous. The greater challenge lies in accurately diagnosing obesity at the right time, reflecting the true increase in visceral fat. BMI may not accurately represent...
14 July 2024 8,771 4 View