I want to dissolve one drug in DMSO but I am wondering about its toxicity on the final concentration on cells?
It is seen that in open field test mice do rearing more suggesting they are more exploratory or curious. Now it is considered one of the markers of anxiety like behaviour. But it is curiosity I...
01 February 2019 1,306 2 View
For the detection of anxiety of mouse which test is more suitable and why? Thanks, MS
01 February 2019 2,962 1 View
I have found my mice showed more rearing and grooming but my question is why they do that more and what does it indicates? Thanks MS
01 February 2019 5,081 7 View
How the hyperactivity of mouse can be measured and how we can understand that these mice are hyperactive? Thanks MS
01 February 2019 1,732 1 View
Mice are naturally nocturnal but most of the lab behavioral tests are conducted in the day time. Is this confounding? Therefore when is the best time to do behavioral test of mice/rodents? Thanks...
31 December 2018 768 4 View
For some reasons most of the time multiple behavioral tests such as open field, rotarod, walking beam, and novel object recognition need to conduct in parallel. However, there may be time issue...
31 December 2018 8,221 3 View
It is observed that oligodendrocytes functions differs based on its position in CNS, why?
31 December 2018 3,543 2 View
Dears, To test anxiety and locomotion commonly "Open filed" and "Elevated-plus maze" apparatus are used. However, for certain reasons multiple instruments is not possible to use in same study. To...
31 December 2018 5,043 4 View
Hello, Is there any drug in the market that can activate B or T cells? Thanks in advance, Monokesh
31 December 2016 2,632 0 View
Can any one suggest the proper antibody to do immuno stain for CD8 cells in 4% PFA fixed mouse brain from anyones own experience that works? Thanks in advance Xx
07 August 2016 6,500 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...
15 July 2024 9,827 1 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View
I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...
24 June 2024 9,319 0 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
12 June 2024 6,087 0 View
Is it possible to do FACS on snap frozen cells ? They were isolated and directly dropped in liquid N2. They were not frozen with DMSO or FBS.
11 June 2024 8,915 0 View
Hello, I discovered that a compound that I use, which get integrated into RNA, might have an unspecific impact on mRNA stability of a gene of interest. To confirm that, I pretreated my cells for...
10 June 2024 276 2 View
I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...
10 June 2024 9,564 6 View