How anxiety can be separated from curiosity in open field test for mice?

It is seen that in open field test mice do rearing more suggesting they are more exploratory or curious. Now it is considered one of the markers of anxiety like behaviour. But it is curiosity I...

01 February 2019 1,157 2 View

Elevated plus maze VS. open field anxiety test?

For the detection of anxiety of mouse which test is more suitable and why? Thanks, MS

01 February 2019 2,780 1 View

Meaning of increased rearing and grooming?

I have found my mice showed more rearing and grooming but my question is why they do that more and what does it indicates? Thanks MS

01 February 2019 4,927 7 View

Hyperactivity in mouse?

How the hyperactivity of mouse can be measured and how we can understand that these mice are hyperactive? Thanks MS

01 February 2019 1,573 1 View

When is the best time to do mice behavior test?

Mice are naturally nocturnal but most of the lab behavioral tests are conducted in the day time. Is this confounding? Therefore when is the best time to do behavioral test of mice/rodents? Thanks...

31 December 2018 509 4 View

How many behavioral tests can be conducted in parallel in mice?

For some reasons most of the time multiple behavioral tests such as open field, rotarod, walking beam, and novel object recognition need to conduct in parallel. However, there may be time issue...

31 December 2018 8,055 3 View

Why oligodendrocytes behave differently in different parts of the CNS?

It is observed that oligodendrocytes functions differs based on its position in CNS, why?

31 December 2018 3,358 2 View

Which is better test to assess anxiety in mouse?

Dears, To test anxiety and locomotion commonly "Open filed" and "Elevated-plus maze" apparatus are used. However, for certain reasons multiple instruments is not possible to use in same study. To...

31 December 2018 4,901 4 View

Activator of B or T cells

31 December 2016 2,492 0 View

Antibody for CD8 T cells for immunohistochemistry/flurescence....

07 August 2016 6,347 2 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

14 February 2021 5,633 1 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View

What are the main conditions suited for synthesizing a PVDF thin film by solvent casting method?

I have been trying to synthesize PVDF thin film by solvent casting method. I started by using 2:3 DMSO: acetone as solvents. The solution was stirred for 3hrs at 60 deg and was dried at 90 degrees...

09 February 2021 8,850 3 View

H2O2 scavenging activity problem??

When I do the test of plant against the free radicals of H2O2 the control had a value about 0.9126 The critical point is when I prepared the extract with DMSO the absorbance appear more than 2...

09 February 2021 6,430 2 View