Hello!

I wanted to know if anyone has ever experienced anything like this. I have tried subcloning erythromycin resistance gene to pCL55-itet vector. I confirmed that subcloning was success. I have streaking cloning DH5-a which get erythromycin resistance gene on LB+50 mg/L erythromycin agar. After 18h on 37 ℃, I check the plate and found both cloning DH5-a harboring erythromycin resistance gene and non-cloning DH5-a survive in erythromycin contained agar plate. I think non-cloning DH5-a couldn't grow on agar plate since It hasn't erythromycin resistance gene. Please help me to why this results happening! For reference, pCL55-itet has ampicillin marker not to erythromycin.

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