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Hello! I wanted to know if anyone has ever experienced anything like this. I have tried subcloning erythromycin resistance gene to pCL55-itet vector. I confirmed that subcloning was success. I...
04 July 2018 2,756 4 View
Hi I try to cut DNA due to cloning to vector. I have to use SmaI and BgIll, they have 100 % activity at cutsmart buffer and NEBuffer, respectively. If I use only one buffer, either smal or BgIll...
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Hello. I'm trying to isolate bacillus sp. form me-ju. I would like to get bacillus sp. by using selective media. I will appreciate if anyone let me know which media is best for!
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I try to design specific primer to distinghuish two speicies. However, their housekeeping gene is highly conserved. It's hard to find polymorphic site. Finally, I found different region. 1....
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