7 Questions 5 Answers 0 Followers
Questions related from Lee Byunghoon
Hello! I have researched about detecting clindamycin resistance gene in B. licheniformis. I tried MIC test and selected clindamycin resistance strain and sensitive strain. After than, I requested...
12 September 2018 6,968 3 View
Hello! I try to make competent cell using Bacillus subtilis ISW1214 labatory strain. I did transformation pUC19 vector to confirm my competent cell efficiency. However, It didn't work well... Here...
09 July 2018 8,576 3 View
Hello! I wanted to know if anyone has ever experienced anything like this. I have tried subcloning erythromycin resistance gene to pCL55-itet vector. I confirmed that subcloning was success. I...
04 July 2018 2,786 4 View
Hi I try to cut DNA due to cloning to vector. I have to use SmaI and BgIll, they have 100 % activity at cutsmart buffer and NEBuffer, respectively. If I use only one buffer, either smal or BgIll...
14 June 2018 7,382 13 View
Hello. I'm trying to isolate bacillus sp. form me-ju. I would like to get bacillus sp. by using selective media. I will appreciate if anyone let me know which media is best for!
22 December 2017 1,724 3 View
Hello! I have researched antibiotics susceptibility test for microbial safety test. I did MIC test for clindamycin and erythromycin. The results show that clindamycin and erythromycin have a...
04 December 2017 6,976 4 View
I try to design specific primer to distinghuish two speicies. However, their housekeeping gene is highly conserved. It's hard to find polymorphic site. Finally, I found different region. 1....
23 November 2017 4,118 3 View
