Lee Byunghoon

5 Questions 5 Answers 0 Followers

Questions related from Lee Byunghoon

Is DH5-a resistant to erythromyhcin??

Hello! I wanted to know if anyone has ever experienced anything like this. I have tried subcloning erythromycin resistance gene to pCL55-itet vector. I confirmed that subcloning was success. I...

04 July 2018 2,760 4 View

How to use restrict enzyme which have different buffer activity?

Hi I try to cut DNA due to cloning to vector. I have to use SmaI and BgIll, they have 100 % activity at cutsmart buffer and NEBuffer, respectively. If I use only one buffer, either smal or BgIll...

14 June 2018 7,319 13 View

Could anyone help me to choos bacilus sp. selective media?

Hello. I'm trying to isolate bacillus sp. form me-ju. I would like to get bacillus sp. by using selective media. I will appreciate if anyone let me know which media is best for!

22 December 2017 1,661 3 View

Does anyone know how to make antibiotics resitance corelaion by data?

Hello! I have researched antibiotics susceptibility test for microbial safety test. I did MIC test for clindamycin and erythromycin. The results show that clindamycin and erythromycin have a...

04 December 2017 6,948 4 View

Can anyone help me to design a specific primer?

I try to design specific primer to distinghuish two speicies. However, their housekeeping gene is highly conserved. It's hard to find polymorphic site. Finally, I found different region. 1....

23 November 2017 4,086 3 View