Best novel method to optimize 3D geometry and its fluid?

Do you have any idea what is the best method to optimize 3D geometry and its fluid without linking the code with fluent (or any flow solver) ? In order to find the best 3d geometry and decrease...

10 April 2020 9,321 1 View

I have problem with cytotoxicity test of gemcitabine.how can i fix it?

Hi, i am working on effects of gemcitabine in HepG2 cell line and i should use the IC50 of Gemcitabine in the experiments.i found different values in literature and the numbers are highly variable...

06 December 2019 6,382 3 View

What is the IC50 of Gemcitabine in HepG2 cell line?

Hi, i am working on effects of gemcitabine in HepG2 cell line and i should use the IC50 of Gemcitabine in the experiments.i found different values in literature and the numbers are highly variable...

26 August 2019 8,545 4 View

In flow analysis in FLAC software, what is the reason for moving the model after the flow analysis in the earth dam?

Hello I have a model of earth dam in FLAC 2D and I did an uncoupled analysis for mechanical and flow. (set mech off/flow on ) Next step, when mech on/flow off ,the model starts to move in the...

14 July 2019 5,146 3 View

Do any researcher have ideas for the best medical Phantom that could be employed in segmating 3D medical data?

I am doing a research for segmenting 3D medical volumes using different techniques I used recently NEMA IEC body phantom and CIRS abdominal phantom,model 057 in my research I am looking for the...

19 May 2019 9,979 2 View

I have problem with designing a primer for my gene,how can i fix it???

Dear all.Hi. i want to design a primer for gene(BCl2) that have multiple variant. And I can not design a primer that includes all my gene variants and also be specific for my gene. what should i...

13 January 2019 2,430 2 View

How can i clone a plasmid?

how can i clone a plasmid that i don't know any thing about its sequence , and the backbone of the plasmid?

05 January 2019 6,028 15 View

Why do i have band in negative control in ITS primer?

I look for variation in strain bacterium and I want to do sequencing but I have a band in negative control that same in my strain bacterium first time I noticed band in my negative control, I...

02 December 2018 5,889 10 View

Hi, i am looking for anionic polyacrylamide or poly(acrylic acid) with charge density around 2 meq/g and low mw of 50000 g/mol?

what chemical suppliers are providing such polymers?

09 September 2018 7,331 1 View

The data of maximum specific growth rate in the continuous biorector versus pH?

Hello Dear all I want to examine the effect of pH on maximum specific growth rate in the continuous biorector for acetic acid production by Acetogenium kivui or Clostridium thermolacticum...

17 October 2017 9,311 1 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Which root in this tap root system, in the picture attached, should be used for root sectioning in order to study the root anatomical details?

There are numerous roots in this tap root system. Kindly suggest or share a research paper where I can clarify the doubts as to which root should be chosen for sectioning and for further root...

14 February 2021 1,816 3 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View