CXCR4 gene.
Are there special method to assess morphological or pathological of Sertoli cells?I need a reliable and accurate method or scale to evaluate the morphology of Sertoli cells in the sections that...
01 February 2017 611 0 View
Pyroptosis is a form of programmed cell death associated with antimicrobial responses during inflammation. Now I want to know about how to detect it?
04 May 2016 5,196 2 View
What is the best staining for the evaluation of testicular tissue?
01 February 2016 5,149 7 View
What is the best fixative for fixing the testicles? How long should the testes remain in the material to achieve the appropriate result?
07 August 2015 7,493 6 View
For exampe: a research about anatomy course, can be useful for probable changes in the educational system?
02 March 2015 4,260 1 View
Pyroptosis is a form of programmed cell death associated with antimicrobial responses during inflammation.
08 September 2014 1,161 4 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
