Dear Ahmad,
A correct question is what is the best fixative, rather than what is the best staining. H&E is quite fine. By the way, testis is an organ and not a tissue.
Best,
Ivan
@ Mohamed
Thanks for your attention to my question.
So right. But I want to know the viewpoint of reproduction or anatomical sciences researchers, which staining is better?
Because many of the points comes with experience.
Dear Mohamed and Ahmed,
My point is the following: testis is a specific organ with relatively fragile structure and is an object of specific fixation techniques (Davidson's fluid, Carnoy etc). Morphology details are revealed by a variety of staining methods too.
Google is fine as search for a term but being a histologist myself, I will not agree with the following idea that a complex structure may be named a tissue. The testicular tissue may refer only to the complex between Sertoli cells and spermatocyte precursors and the basal membrane of the seminiferous tubuli. If that is the object of research, my apologies, didn't get the point. And it falls in the definition of tissue: same origin and distinct function, right?
Second, the testis as organ is stained, because normally, one isolates pieces of testis for staining and no seminiferous tubuli only. There are several simple reasons for that: time consuming, a lot of structure damage and definitely too expensive t. So in the end the organ is fixed processed, cut and stained. Saying again that testicles are quite a fragile organ, this will be the way to analyze the "testicular tissue" in detail with the lowest risk for artefacts...
The discussion is endless, I just wanted to push it and to help!
Attached is an image of a mouse testis fixed with modified Davidson's fixative, cut at 2 um thickness and stained in H&E. If that is the thing you want to achieve, Ahmed, you're welcome to ask for "tips and tricks". I will send you everything I have in terms of protocols, procedures, etc
Regards,
Ivan
Hello
i agree with all researchers , look at these links hope help you
Good Luck
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0272.1996.tb02754.x/abstract
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2677676/
http://www.leicabiosystems.com/fileadmin/biosystems/whitepapers/95.8012_rev_a_assessing_the_quality_of_tissue_processing_email.pdf
http://library.med.utah.edu/WebPath/HISTHTML/STAINS/STAINS.html
Chapter Histological Methods for the Evaluation of the Testis
Dear Dr. Ivan Kanchev
very good you are really expert in reproductive system histology ana pathology. I agree with your point that testis is an organ and not tissue and after you made tissue sections then you can said this is a testicular tissue. we should differentiate between tissues and organs( before we make sectioning that is organ after we make sectioning and staining that is tissues)
Dear Ahmed
there is no best staining but there is routin stain and special stain used depends on your objectives depends on what you are looking for, depends on experimental design.
So you must foucus on your objective and make it clear to know what you want. bu the way Dr. Ivan I know him very well and i contact him many times and help me more than other researchers, he is very expert in sex organ patthology, do not hasitate to ask him any question he is very very kind and helpful and friendly researchers with high respect to others.
good luck
Ali
Dear Ali,
Thank you for your nice words. I don't consider myself an expert but just a person trying to stand certain ground in terms of terminology and precision. There are much more experienced people than me in the field. I know that sometimes I do push the things a bit more but I also try to help people as much as I can. The discussion becomes interesting...
Best,
Ivan
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