Pyroptosis is a form of programmed cell death associated with antimicrobial responses during inflammation.
You can use a reagent such as caspase-1-specific FLICA from Immunochemistry to observe the assembly of speck-like inflammasome structures combined with cleavage of caspase-1 and IL-1beta by Western blot and release of IL-1beta in the supernatant by ELISA or Western blot. You could also stain for one of the NLR protein sensors or ASC to observe the inflammasome, as well. Finally, you can use the YVAD inhibitor peptide to see if cell death (as measured by a variety of methods) is caspase-1-dependent, which is considered classical for pyroptosis.
You can use a reagent such as caspase-1-specific FLICA from Immunochemistry to observe the assembly of speck-like inflammasome structures combined with cleavage of caspase-1 and IL-1beta by Western blot and release of IL-1beta in the supernatant by ELISA or Western blot. You could also stain for one of the NLR protein sensors or ASC to observe the inflammasome, as well. Finally, you can use the YVAD inhibitor peptide to see if cell death (as measured by a variety of methods) is caspase-1-dependent, which is considered classical for pyroptosis.
To appropriately distinguish between pyroptosis vs apoptosis you should incorporate reagents that detect active Caspase 1, active Caspase 3/7, Annexin, and Propidium Iodide.
I think that the responses should be "included" in one of the three attached articles.
Best regards
Robert
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