I want to conduct click reaction with azide in Fmoc-SPPS.
I want to know more about Uranium ore deposits in world.
11 August 2024 6,720 0 View
I want to know more about diamond ore deposits in world.
11 August 2024 2,167 1 View
We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
10 August 2024 6,678 14 View
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,198 5 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
What are a “Farmers Producer Organization” (FPO) and its essential features?
10 August 2024 476 5 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
08 August 2024 4,831 4 View
How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
08 August 2024 8,161 0 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
I did some column tests in my lab where I needed to detect the DOC fractions using LC-OCD. For the abiotic columns I used sodium azide to restrict the biofilm formation. I did LC-OCD to detect the...
07 July 2024 468 0 View
Hey everyone! I'm doing peptide synthesis on Rink amide resin and sometimes I need to add amino acids with secondary amines or beta amino acids. Normally I synthesize 35 to 40 mer peptides. As a...
21 May 2024 9,035 0 View
In order to proceed with the quantification of an Azide Alkyne Cycloaddition reaction efficiency by using a fluorogenic probe with Cy5-DBCO, I first need to construct a calibration curve. The...
16 May 2024 6,266 5 View
I am planning to synthesize a peptide :YSTCDFIM (MW 978). However, no matter how I did the synthesis, the LCMS result always showed peaks at 529, 644, 791, 1035. It seems like I always missed STDF...
10 March 2024 4,918 3 View
Dear Peptide Chemists, in SPPS we forgot to deprotect the Fmoc protection group of the N-term on a complicated enough product. It's not cleaved from the resin with standard TFA protocol, washed...
27 February 2024 3,700 1 View
It always need to functionalize a secondary alcohol with leaving group (e.g. OTf, OMs), nucleophilic substitution of the leaving group to afford an OH with configuration inversions , and then...
26 October 2023 9,722 5 View
I am performing azide-alkyne cycloaddition using a propargylated small molecule (alkyne) which is getting incorporated into proteins and biotin azide for pulldown by strepavidin beads. I am seeing...
24 October 2023 536 1 View
I am working with a polymer that has azide groups, and I would like to click it with fluorescent dye that also has azide group. My idea was to use DBCO-PEG2-DBCO for conjugation this 2 azido...
19 October 2023 9,680 3 View
We doing this as a high school project of BOD on pond water and are following a video method (https://www.youtube.com/watch?v=QZXlIbeGWA0) we found on the internet and we get a pale brown ppt...
27 September 2023 8,477 1 View
