How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How long can synthesized peptides using SPPS be stored? Storage Conditions?

I have been synthesizing peptides on resin beads. I am wondering what best storage method is there to store the beads after synthesis before cleaving. And how long can they be stored in that...

02 February 2021 5,636 5 View

Proper RP-HPLC workup following peptide synthesis?

Hello, I've recently synthesized a short (17aa) peptide via manual SPPS. I was instructed to submit a small pipette tip's worth of material onto a capillary column to obtain a rough estimate of...

12 January 2021 9,432 3 View

Any Custom Peptide Synthesis Companies In India? Custom Peptides Manufacturing Lab in India?

We are looking for many Custom Peptides in India, with good quality for our Bio-pharmaceutical research. Please suggest us best Custom Peptide Synthesis and Service Providers from India? Expecting...

31 December 2020 6,516 7 View

SPPS: peptide failure to elongate?

SPPS of a 16 aa peptide was performed on a 0.15 mmol scale. Resin gained a minimal amount of weight: starting weight of 224 mg and a final weight of 280.I believe my peptide failed to elongate but...

21 December 2020 9,428 11 View

Help with gold nanoparticle with nile blue, why sodium azide???

Hello! I have been trying to understand this for more than a year now, and I am at a loss. Can anyone help, please?? So, I follow this protocol :Statistical Correlation Between SERS Intensity...

31 May 2020 658 10 View

What kind of Na azide for Flow Cytometry experiment?

I need to use NaN3 as a blocking reagent to prevent cells from capping and internalizing. What strength do I buy it at and what concentration should I use it at? Does anyone have a protocol for...

17 February 2020 8,880 3 View

What kind of Na azide for Flow Cytometry experiment?

I need to use NaN3 as a blocking reagent to prevent cells from capping and internalizing. What strength do I buy it at and what concentration should I use it at? Does anyone have a protocol for...

01 February 2020 1,882 2 View

Hey! PLease help me out: do yoou know what happens if you add more Sodium azide in your primary antibody dilution? f.ex. 0,1%? any experience?

will the antibody binding be affected? (recommended is 0,02-0,05%) i would be happy to get some help

30 January 2020 7,624 1 View

Hey! PLease help me out: do yoou know what happens if you add more Sodium azide in your primary antibody dilution? f.ex. 0,1%? any experience?

will the antibody binding be affected? (recommended is 0,02-0,05%) i would be happy to get some help

31 December 2019 2,994 1 View

Optimal Conditions for DBCO-azide Reaction?

I was trying to conjugate dye and PEG onto DBCO functionalized nanoparticles. The dye I use is azide-Cy5 and the PEG is azido-PEG24-NHS. I first react the dye with particles for 1 whole day...

09 October 2019 5,121 3 View