Hello,
I want to work with Resveratrol against some cancer cells. I want to know your experience about its Non-toxic dose by using MTT, its solubilty and some other things. Please let me know if someone is working with it. Thanks.
First of all which cacer cell line you are interested because evry cell line has different doses as IC-50 values. And please go to pubmed data with resvertrol and cancer key words you get thousands of publication.
Consideration for safety and potential mechanisms of activity following multiple dosing of RSV, I hope that will help: "Patel KR et al. Clinical trials of resveratrol. Ann N Y Acad Sci. 2011 Jan;1215:161-9".
Perhaps this very recent paper is of interest if you are considering to use resveratrol on cancer cells: http://www.nature.com/news/chemistry-chemical-con-artists-foil-drug-discovery-1.15991.
It certainly made me reconsider some work that I had been planning.
Good luck with your endeavours!
I suggest you to take a look on the publications of Pr. Dominique Delmas who published pioneer work on resveratrol effects on cancer cells. You might also contact him at the following adress: [email protected].
Feel free to specifiy that I suggest you to contact him.
David
David Wendehenne, Thank you very much, you information is very useful for me,
Best Regards!
Hi Salman. I have treated 2 different endometrial cancer cells with Resveratrol and evaluated cell viability with MTT (48-72h post treatment). I found the IC50 to be about 50uM and all cells were dead at 250uM. This should give you a ballpark range to start with. Hope this helps!
What type of cancer cells? Resveratrol is a phytoestrogen, u have to check the presence of estrogen receptor in your cancer cell lines. I haven't work with resveratrol but with other phytoestrogen and 10uM works well to inhibits cell growth. Best
Hi, I worked with RV on oral cancer lines. In our experiments the range of concentrations needed for inducing the inhibition of growth was 100uM. Here, there is our publication: Oral Oncol. 2010 Jan;46(1):42-8
We tried also other oral cancer cell lines (HSC2, CAL27, AT84, ...) with similar results
Best regards!
Hi, I tested resveratrol effects on several different cell lines and on normal cells. I suggest you to use RSV concentrations ranging from 1 to 50-100 uM and to analyze the effects by evaluating cytotoxicity, cell proliferation rate and cell cycle effects (by FACS analysis). You may find more information in my papers on this issue.
Hoping this help,
best
Have a look this paper: Neurons from senescence-accelerated SAMP8 mice are protected against frailty by the sirtuin 1 promoting agents melatonin and resveratrol.
http://www.ncbi.nlm.nih.gov/pubmed/22085194
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