Is anyone willing to share the AY27 rat bladder cell line?

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What are good polymers for acoustic tissue phantoms?

Hello community, I need a rubbery material which would not generate significant acoustic reflections when immersed in an aqueous medium. Speed of sound in PDMS is 1000 m/s, so there will be a...

02 March 2019 2,326 3 View

What are good solvents for Trichloro(1H,1H,2H,2H-perfluorooctyl)silane?

I want to treat PDMS channels with it, but condensation does not treat the whole length of the channels. The only information I have is that the silane reacts violently with water. All of the...

10 November 2016 1,486 10 View

How can I reduce the surface roughness of SU8 structures?

Hello all, To be specific, I want to reduced the surface roughness of the sidewalls of SU8. I attached a picture to help everyone understand what I mean. This is a side view of a 125 um high...

08 September 2016 7,081 5 View

Total RNA from a U87 cell - any numbers?

Does anyone have numbers? Either average amount from a single cell or per colony. I couldn't find anyone reporting exactly that in a paper. Thanks.

04 May 2016 8,578 2 View

What gels for cell culture, biological or not, have you heard of that melt at 50-60 degrees C?

I am thinking of trying a LMP agarose, after adding a protein solution in PBS, and incubation at 4 degrees overnight. There is also polycaprolactone which seems to require quite some chemicals...

07 August 2015 7,796 5 View

Is a High NA objective and TEM00 beam normal?

Consider a single mode Gaussian beam entering a 0.75 NA objective. I made measurements of beam's FWHM around the focus. It looked like before the focus, the beam converges according to NA=0.75....

05 June 2015 6,661 1 View

Any ideas about cell culture plates from unusual materials?

I need to glue a functionalized coverslip with Valap to the bottom of a chamber. Coverslips are 22x22 mm, #72198-10, from EMS. 1. Does anyone know of commercial products available for that size? I...

02 March 2015 2,282 9 View

What are the chromophores with the highest absorption that you know?

I am interested in the green part of the spectrum. Please suggest organic molecules and nanoparticles other than gold. Thanks!

06 July 2014 3,849 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View