What are good polymers for acoustic tissue phantoms?

Hello community, I need a rubbery material which would not generate significant acoustic reflections when immersed in an aqueous medium. Speed of sound in PDMS is 1000 m/s, so there will be a...

02 March 2019 2,271 3 View

What are good solvents for Trichloro(1H,1H,2H,2H-perfluorooctyl)silane?

I want to treat PDMS channels with it, but condensation does not treat the whole length of the channels. The only information I have is that the silane reacts violently with water. All of the...

10 November 2016 1,420 10 View

How can I reduce the surface roughness of SU8 structures?

Hello all, To be specific, I want to reduced the surface roughness of the sidewalls of SU8. I attached a picture to help everyone understand what I mean. This is a side view of a 125 um high...

08 September 2016 6,957 5 View

Total RNA from a U87 cell - any numbers?

Does anyone have numbers? Either average amount from a single cell or per colony. I couldn't find anyone reporting exactly that in a paper. Thanks.

04 May 2016 8,508 2 View

What gels for cell culture, biological or not, have you heard of that melt at 50-60 degrees C?

07 August 2015 7,720 5 View

Is a High NA objective and TEM00 beam normal?

Consider a single mode Gaussian beam entering a 0.75 NA objective. I made measurements of beam's FWHM around the focus. It looked like before the focus, the beam converges according to NA=0.75....

05 June 2015 6,602 1 View

Any ideas about cell culture plates from unusual materials?

I need to glue a functionalized coverslip with Valap to the bottom of a chamber. Coverslips are 22x22 mm, #72198-10, from EMS. 1. Does anyone know of commercial products available for that size? I...

02 March 2015 2,217 9 View

What are the chromophores with the highest absorption that you know?

I am interested in the green part of the spectrum. Please suggest organic molecules and nanoparticles other than gold. Thanks!

06 July 2014 3,784 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View