What are good polymers for acoustic tissue phantoms?

Hello community, I need a rubbery material which would not generate significant acoustic reflections when immersed in an aqueous medium. Speed of sound in PDMS is 1000 m/s, so there will be a...

02 March 2019 2,271 3 View

What are good solvents for Trichloro(1H,1H,2H,2H-perfluorooctyl)silane?

I want to treat PDMS channels with it, but condensation does not treat the whole length of the channels. The only information I have is that the silane reacts violently with water. All of the...

10 November 2016 1,420 10 View

How can I reduce the surface roughness of SU8 structures?

Hello all, To be specific, I want to reduced the surface roughness of the sidewalls of SU8. I attached a picture to help everyone understand what I mean. This is a side view of a 125 um high...

08 September 2016 6,957 5 View

Total RNA from a U87 cell - any numbers?

Does anyone have numbers? Either average amount from a single cell or per colony. I couldn't find anyone reporting exactly that in a paper. Thanks.

04 May 2016 8,508 2 View

What gels for cell culture, biological or not, have you heard of that melt at 50-60 degrees C?

07 August 2015 7,720 5 View

Is a High NA objective and TEM00 beam normal?

Consider a single mode Gaussian beam entering a 0.75 NA objective. I made measurements of beam's FWHM around the focus. It looked like before the focus, the beam converges according to NA=0.75....

05 June 2015 6,602 1 View

Any ideas about cell culture plates from unusual materials?

I need to glue a functionalized coverslip with Valap to the bottom of a chamber. Coverslips are 22x22 mm, #72198-10, from EMS. 1. Does anyone know of commercial products available for that size? I...

02 March 2015 2,217 9 View

Is anyone willing to share the AY27 rat bladder cell line?

We are located in Toronto, Ontario. I would appreciate your help.

08 September 2014 2,513 2 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View