What are good polymers for acoustic tissue phantoms?

Hello community, I need a rubbery material which would not generate significant acoustic reflections when immersed in an aqueous medium. Speed of sound in PDMS is 1000 m/s, so there will be a...

02 March 2019 2,271 3 View

What are good solvents for Trichloro(1H,1H,2H,2H-perfluorooctyl)silane?

I want to treat PDMS channels with it, but condensation does not treat the whole length of the channels. The only information I have is that the silane reacts violently with water. All of the...

10 November 2016 1,420 10 View

How can I reduce the surface roughness of SU8 structures?

Hello all, To be specific, I want to reduced the surface roughness of the sidewalls of SU8. I attached a picture to help everyone understand what I mean. This is a side view of a 125 um high...

08 September 2016 6,957 5 View

What gels for cell culture, biological or not, have you heard of that melt at 50-60 degrees C?

07 August 2015 7,720 5 View

Is a High NA objective and TEM00 beam normal?

Consider a single mode Gaussian beam entering a 0.75 NA objective. I made measurements of beam's FWHM around the focus. It looked like before the focus, the beam converges according to NA=0.75....

05 June 2015 6,602 1 View

Any ideas about cell culture plates from unusual materials?

I need to glue a functionalized coverslip with Valap to the bottom of a chamber. Coverslips are 22x22 mm, #72198-10, from EMS. 1. Does anyone know of commercial products available for that size? I...

02 March 2015 2,217 9 View

Is anyone willing to share the AY27 rat bladder cell line?

We are located in Toronto, Ontario. I would appreciate your help.

08 September 2014 2,513 2 View

What are the chromophores with the highest absorption that you know?

I am interested in the green part of the spectrum. Please suggest organic molecules and nanoparticles other than gold. Thanks!

06 July 2014 3,784 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View