Is anyone looking at photoreceptor degeneration in rats following exposure to high intensity light?

More Philippe D'Onofrio's questions See All

Coulkd you help finding a paper about Bryophytes ?

Some monthes ago on RG I found a nice paper by a bryologist explaining that in a single small tuff of epiphytic mosses different species might co-occur. I remember a plate illustrating type...

01 July 2024 5,102 0 View

What tag to use inside the sequence of an hydrophobic transmembrane protein ?

Hi everyone, I want to study the translation efficiency of multiple version of the same protein coded by different genotypes. To do so I need to conserve the translation initiation context ->...

29 April 2024 6,779 1 View

What if all multilayered plastic packagings could become recyclable? Come to the keynote of P Shahgaldian at # ISPTS 2024?

plastic revolution?

10 April 2024 4,191 2 View

What is the shortest sequence encoding a fluorescent protein ?

Hi everyone, everything is in the title question ! To the best of your knowledge, what is the shortest sequence you know that translate into a fluorescent protein that could be used for flow...

07 March 2024 1,762 1 View

Who has experience with transfecting HaCaT keratinocytes ?

Hi everyone, I'm currently working on the HaCaT keratinocyte cell line. I have them in culture under low and high calcium concentration to keep them undifferenciated or differenciated,...

04 March 2024 2,153 0 View

Why the concentration of my expression vector drop with time and how to stop it ?

Hello everyone, I use several pcDNA3.1 expression vectors to transfect cells. The vectors were prepared by midi-prep a year ago and diluted in TE buffer. Now that I run new experiments, I...

04 March 2024 1,867 3 View

MODFLOW: How to efficiently modify existing 3DGrid using GMS?

I work on a MODFLOW Model using GMS to simulate subsurface water exchanges between a small straightened stream and its heavily modified alluvial plain. I’m looking for efficient methods to modify...

09 January 2024 708 0 View

How to get rid of chelex resin before 0.22µm filtration ?

Hello everyone, I'm curently working on HaCaT cells which require low calcium medium to return to undifferentiate state. To do so, I need to chelate my FBS for 1h with chelex resin among other...

12 December 2023 4,975 2 View

Can you share scientific literature or research experience about building a timeline with participants with interviews?

In an action-research, our research team is conducting interviews to identify key moments about professional practices in the field of education with participants.

04 December 2023 2,114 1 View

Do FAP natural inhibitors exist ?

Hello, I was not able to find a natural inhibitor for Fibroblast Activation Protein (FAP) in the banks or in the literature. Even among broad spectrum inhibitors Is there any identified? Thank...

26 November 2023 636 2 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Alain M Bron

Yes Manual Vidal Sanz did a great job on this topic. Browse his name on internet and his papers and you will easily find his email address to contact him. Tell him that I recommended him to you. Manual is located in Murcia, Spain.

Kind regards

Alain Bron, Dijon, France

Philippe D'Onofrio

Thank you very much, Alain. Your recommendation was very helpful.