Who can tell me what these letters refer to, it's frequently used in the primer sequences designated universal 16 S (R-M-K-N-W...........) ?
For example:
27F (AGAGTTTGATCMTGGCTCAG)
Thanks
These letters show us the degenerate primers. For example if you have an amino acid sequence for a special gene, the amino acid code W corresponds to trytophan amino acid . But if this W is in the codon of the amino acid sequence it means that in that position the bases are A and T together. The primer is synthesized both in the A and T format. This will help us to our primers can bind places that has both A or T in that position. You can also see here: http://dps.plants.ox.ac.uk/langdalelab/protocols/PCR/degenerate_primer.pdf
These letters show us the degenerate primers. For example if you have an amino acid sequence for a special gene, the amino acid code W corresponds to trytophan amino acid . But if this W is in the codon of the amino acid sequence it means that in that position the bases are A and T together. The primer is synthesized both in the A and T format. This will help us to our primers can bind places that has both A or T in that position. You can also see here: http://dps.plants.ox.ac.uk/langdalelab/protocols/PCR/degenerate_primer.pdf
Hi Meriem,
Check IUPAC code for nucleotides here http://www.bioinformatics.org/sms/iupac.html.
R = A + G
Y = C + T
M = A + C
K = G + T
S = G + C
W = A + T
H = A + T + C
D = G + A + T
B = G + T + C
V = G + A + C
N = A + T + G + C
Hi!
I agree with all, however Chiara is making it easy for you.
Good luck.
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