Is anyone familiar with Type I collagen detection in Western blots?

Venugopal Rao Mittapalli @Venugopal_Mittapalli

03 March 2014 1 2K Report

Can anyone tell me one good buffer which works best to isolate type I collagen from murine skin samples to detect it through western blots?

I have homogenized skin tissue and lysed in NP40 (normal lysis buffer) and also boiled directly the skin homegenate in BSB, in both the cases i could not see Type I collagen bands. Please suggest.

Badges
Science method

More Venugopal Rao Mittapalli's questions See All

How to reduce nsSNVs from WES data ?

We performed Whole Exome sequencing (WES) on some patient samples of a well known disease. Our bioinformaticians gave us the data in XL sheets with 300 plus genes with deletions, insertions and...

01 February 2018 6,558 2 View

Bioinformatic databases on Human Haematopoetic stem cells?

We have generated RNA-SEQ data from patient samples. We would like to analyse this data in human haematopoetic stem cell databases. We tried Reactome.org but we could not find many genes...

10 November 2016 2,472 6 View

Does the presence of TGFb in serum induce fibrosis in organs?

We know that TGFb is a profibrotic factor. Does the presence of TGFb in serum induce fibrosis in organs? Can anyone suggest an article where it shows the presence of high levels of TGFb in serum...

03 April 2014 3,331 2 View

Is it possible to make protein lysates from organotypic co-cultures?

Hi, I am working with 3D organotypic co-cultures (skin equivalents), using human keratinocytes and fibroblasts. I can section these cultures and make immunflourescene stainings with antibodies but...

01 February 2014 172 4 View

I would like to know a marker (s) that stain metastatic cells in the lung

I would like to know a marker (s) that stain metastatic cells in the lung. Carcinomas developed on mice skin (SCC) after tumor induction with chemical carcinogens. We found lung metastasis when...

10 November 2013 8,993 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View