Is anyone familiar with Type I collagen detection in Western blots?

Venugopal Rao Mittapalli @Venugopal_Mittapalli

03 March 2014 1 2K Report

Can anyone tell me one good buffer which works best to isolate type I collagen from murine skin samples to detect it through western blots?

I have homogenized skin tissue and lysed in NP40 (normal lysis buffer) and also boiled directly the skin homegenate in BSB, in both the cases i could not see Type I collagen bands. Please suggest.

Clara Ibel Chamorro

Wich conditions do you use for the transfer of proteins in your western?

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