How to reduce nsSNVs from WES data ?

We performed Whole Exome sequencing (WES) on some patient samples of a well known disease. Our bioinformaticians gave us the data in XL sheets with 300 plus genes with deletions, insertions and...

01 February 2018 6,558 2 View

Does the presence of TGFb in serum induce fibrosis in organs?

We know that TGFb is a profibrotic factor. Does the presence of TGFb in serum induce fibrosis in organs? Can anyone suggest an article where it shows the presence of high levels of TGFb in serum...

03 April 2014 3,331 2 View

Is anyone familiar with Type I collagen detection in Western blots?

Can anyone tell me one good buffer which works best to isolate type I collagen from murine skin samples to detect it through western blots? I have homogenized skin tissue and lysed in NP40...

02 March 2014 1,524 1 View

Is it possible to make protein lysates from organotypic co-cultures?

Hi, I am working with 3D organotypic co-cultures (skin equivalents), using human keratinocytes and fibroblasts. I can section these cultures and make immunflourescene stainings with antibodies but...

01 February 2014 172 4 View

I would like to know a marker (s) that stain metastatic cells in the lung

I would like to know a marker (s) that stain metastatic cells in the lung. Carcinomas developed on mice skin (SCC) after tumor induction with chemical carcinogens. We found lung metastasis when...

10 November 2013 8,993 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View