Venugopal Rao Mittapalli

6 Questions 10 Answers 0 Followers

Questions related from Venugopal Rao Mittapalli

How to reduce nsSNVs from WES data ?

We performed Whole Exome sequencing (WES) on some patient samples of a well known disease. Our bioinformaticians gave us the data in XL sheets with 300 plus genes with deletions, insertions and...

02 February 2018 6,614 2 View

Bioinformatic databases on Human Haematopoetic stem cells?

We have generated RNA-SEQ data from patient samples. We would like to analyse this data in human haematopoetic stem cell databases. We tried Reactome.org but we could not find many genes...

11 November 2016 2,528 6 View

Does the presence of TGFb in serum induce fibrosis in organs?

We know that TGFb is a profibrotic factor. Does the presence of TGFb in serum induce fibrosis in organs? Can anyone suggest an article where it shows the presence of high levels of TGFb in serum...

04 April 2014 3,382 2 View

Is anyone familiar with Type I collagen detection in Western blots?

Can anyone tell me one good buffer which works best to isolate type I collagen from murine skin samples to detect it through western blots? I have homogenized skin tissue and lysed in NP40...

03 March 2014 1,578 1 View

Is it possible to make protein lysates from organotypic co-cultures?

Hi, I am working with 3D organotypic co-cultures (skin equivalents), using human keratinocytes and fibroblasts. I can section these cultures and make immunflourescene stainings with antibodies but...

02 February 2014 220 4 View

I would like to know a marker (s) that stain metastatic cells in the lung

I would like to know a marker (s) that stain metastatic cells in the lung. Carcinomas developed on mice skin (SCC) after tumor induction with chemical carcinogens. We found lung metastasis when...

11 November 2013 9,038 3 View