Atypical polyproline recognition by the CMS N-terminal Src homology 3 domain epitope tag is used that is associated interaction partner identified by Western blot. Cells are fixed and processed for immunohistochemistry using M5 and 2166.The slides are processed by an observer blind to the treatment conditions. One thousand Flag-positive on each microslip are  examined for the expression of diffuse htt and htt bodies using

a fluorescence microscope with 60 objective and an ocular grid (Eclipse TE 300; Nikon, Tokyo, Japan). The data are  statistically analyzed with Student’s t test or ANOVA and presented in bar figures as the number of cells with htt bodies per 1000 cells expressing exogenous htt. You can attach polyproline tag for recognitionpurpose on CMS N-terminal