Is anyone familiar with plasmid DNA staining with SYBR green?

More Zaheer Ahmed's questions See All

How do I measure zeta size of a complex containing calcium chloride- and tamra- labelled cationic plasmid dna using zetasizer?

I want to optimize the parameters needed to obtain the exact size of the nanoparticles?

31 December 2014 8,854 0 View

Is there anyone who has dealt with TAMRA detection by flow cytometry?

My peptide is labelled with TAMRA. I want to deliver that TAMRA coupled peptide to the cell lines and then i want to check the fluorescence by FACS. How much micro moles of peptide-tamra would i...

07 August 2014 3,998 3 View

How do you label cell penetration peptide?

I want to label my cell penetration peptide with low molecular weight RED fluorophore. I will analyze my samples with FACS. I am also curious about which terminal of peptide is should label such...

06 July 2014 7,563 1 View

How do you couple the quinoline functional group to the c terminal of a peptide?

I am interested in coupling quinoline as a functional group to the c terminal of my peptide with the idea that quinoline can help in endosomal escape. Please suggest some chemistry for the...

06 July 2014 3,831 7 View

Is it possible to form an amide bond between two proteins by amino-carboxyl group interaction?

I am looking for making an interaction of two proteins invitro using this strategy. Please let me know about some couplers or any alternate way of coupling two proteins or peptides using this...

04 May 2014 6,671 9 View

Can I have a protocol for eluting my HIS tagged protein using EDTA?

04 May 2014 6,839 6 View

Does anybody have experience with HSP-90 alpha?

What exactly is the role of HSP-90 in extracellular environment of the cell? I am wondering whether hsp90 is involved in the translocation of the client protein from outside to inside of the cell....

04 May 2014 6,170 2 View

Why is my HIS tagged peptide-tomato recombinant protein not released from nickle beads in the column after adding elution buffer?

The other fusion proteins are eluted well. But there is something wrong with only one protein. It is expressed well in 500 ml LB with 0.2mM IPTG, overnight incubation at 20C and 150rpm. The...

04 May 2014 3,256 10 View

Can somebody help me find some tools for in-silco membrane-peptide interaction?

I am interested in finding some bioinformatics tools to study how peptides could interact with the cell membrane and what the outcome of this interaction could be. Please help in this regard.

04 May 2014 814 1 View

How can I evaluate the penetration capability of a cell penetrating peptide in silico?

I want to construct my own cell penetrating peptide for which I want to evaluate their capability of penetrating the plasma membrane. Please share some authenticated and cited online tools which...

04 May 2014 8,899 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Tomáš Hluska

You mean to mix the DNA with plasmid, transform (bacterial?) cells and see them under microscope to confirm transformation?

Don't you have any better way?

Kamal Kumar

I don't think it is possible to detect 2-3 plasmids transformed in a cell with the method you are asking for.

Marzieh Mahdavipour

Hi Zaheer

Like other dear friends, I don’t think so.

I admire your idea for trying novel sights of research, and I wish you all the best.

Good luck