Is anyone familiar with plasmid DNA staining with SYBR green?

More Zaheer Ahmed's questions See All

How do I measure zeta size of a complex containing calcium chloride- and tamra- labelled cationic plasmid dna using zetasizer?

I want to optimize the parameters needed to obtain the exact size of the nanoparticles?

31 December 2014 8,806 0 View

Is there anyone who has dealt with TAMRA detection by flow cytometry?

My peptide is labelled with TAMRA. I want to deliver that TAMRA coupled peptide to the cell lines and then i want to check the fluorescence by FACS. How much micro moles of peptide-tamra would i...

07 August 2014 3,944 3 View

How do you couple the quinoline functional group to the c terminal of a peptide?

I am interested in coupling quinoline as a functional group to the c terminal of my peptide with the idea that quinoline can help in endosomal escape. Please suggest some chemistry for the...

06 July 2014 3,784 7 View

How do you label cell penetration peptide?

I want to label my cell penetration peptide with low molecular weight RED fluorophore. I will analyze my samples with FACS. I am also curious about which terminal of peptide is should label such...

06 July 2014 7,483 1 View

Is it possible to form an amide bond between two proteins by amino-carboxyl group interaction?

I am looking for making an interaction of two proteins invitro using this strategy. Please let me know about some couplers or any alternate way of coupling two proteins or peptides using this...

04 May 2014 6,627 9 View

Can I have a protocol for eluting my HIS tagged protein using EDTA?

04 May 2014 6,782 6 View

Does anybody have experience with HSP-90 alpha?

What exactly is the role of HSP-90 in extracellular environment of the cell? I am wondering whether hsp90 is involved in the translocation of the client protein from outside to inside of the cell....

04 May 2014 6,117 2 View

Why is my HIS tagged peptide-tomato recombinant protein not released from nickle beads in the column after adding elution buffer?

The other fusion proteins are eluted well. But there is something wrong with only one protein. It is expressed well in 500 ml LB with 0.2mM IPTG, overnight incubation at 20C and 150rpm. The...

04 May 2014 3,210 10 View

Can somebody help me find some tools for in-silco membrane-peptide interaction?

I am interested in finding some bioinformatics tools to study how peptides could interact with the cell membrane and what the outcome of this interaction could be. Please help in this regard.

04 May 2014 763 1 View

How can I evaluate the penetration capability of a cell penetrating peptide in silico?

I want to construct my own cell penetrating peptide for which I want to evaluate their capability of penetrating the plasma membrane. Please share some authenticated and cited online tools which...

04 May 2014 8,855 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Tomáš Hluska

You mean to mix the DNA with plasmid, transform (bacterial?) cells and see them under microscope to confirm transformation?

Don't you have any better way?

Kamal Kumar

I don't think it is possible to detect 2-3 plasmids transformed in a cell with the method you are asking for.

Marzieh Mahdavipour

Hi Zaheer

Like other dear friends, I don’t think so.

I admire your idea for trying novel sights of research, and I wish you all the best.

Good luck