05 May 2014 2 6K Report

What exactly is the role of HSP-90 in extracellular environment of the cell? I am wondering whether hsp90 is involved in the translocation of the client protein from outside to inside of the cell. If somebody is having some references please share with me. I am very curious about this molecule.

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How do I measure zeta size of a complex containing calcium chloride- and tamra- labelled cationic plasmid dna using zetasizer?

I want to optimize the parameters needed to obtain the exact size of the nanoparticles?

31 December 2014 8,806 0 View

Is anyone familiar with plasmid DNA staining with SYBR green?

I want to stain plasmid DNA with sybr green so that I could detect it after cellular delivery. Is it possible? If yes how would I use sybr green?

11 December 2014 5,577 3 View

Is there anyone who has dealt with TAMRA detection by flow cytometry?

My peptide is labelled with TAMRA. I want to deliver that TAMRA coupled peptide to the cell lines and then i want to check the fluorescence by FACS. How much micro moles of peptide-tamra would i...

07 August 2014 3,944 3 View

How do you couple the quinoline functional group to the c terminal of a peptide?

I am interested in coupling quinoline as a functional group to the c terminal of my peptide with the idea that quinoline can help in endosomal escape. Please suggest some chemistry for the...

06 July 2014 3,784 7 View

How do you label cell penetration peptide?

I want to label my cell penetration peptide with low molecular weight RED fluorophore. I will analyze my samples with FACS. I am also curious about which terminal of peptide is should label such...

06 July 2014 7,483 1 View

Is it possible to form an amide bond between two proteins by amino-carboxyl group interaction?

I am looking for making an interaction of two proteins invitro using this strategy. Please let me know about some couplers or any alternate way of coupling two proteins or peptides using this...

04 May 2014 6,627 9 View

Can I have a protocol for eluting my HIS tagged protein using EDTA?

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04 May 2014 6,782 6 View

Why is my HIS tagged peptide-tomato recombinant protein not released from nickle beads in the column after adding elution buffer?

The other fusion proteins are eluted well. But there is something wrong with only one protein. It is expressed well in 500 ml LB with 0.2mM IPTG, overnight incubation at 20C and 150rpm. The...

04 May 2014 3,210 10 View

Can somebody help me find some tools for in-silco membrane-peptide interaction?

I am interested in finding some bioinformatics tools to study how peptides could interact with the cell membrane and what the outcome of this interaction could be. Please help in this regard.

04 May 2014 763 1 View

How can I evaluate the penetration capability of a cell penetrating peptide in silico?

I want to construct my own cell penetrating peptide for which I want to evaluate their capability of penetrating the plasma membrane. Please share some authenticated and cited online tools which...

04 May 2014 8,855 5 View

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