How do I measure zeta size of a complex containing calcium chloride- and tamra- labelled cationic plasmid dna using zetasizer?

01 January 2015 0 9K Report

I want to optimize the parameters needed to obtain the exact size of the nanoparticles?

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Is anyone familiar with plasmid DNA staining with SYBR green?

I want to stain plasmid DNA with sybr green so that I could detect it after cellular delivery. Is it possible? If yes how would I use sybr green?

11 December 2014 5,626 3 View

Is there anyone who has dealt with TAMRA detection by flow cytometry?

My peptide is labelled with TAMRA. I want to deliver that TAMRA coupled peptide to the cell lines and then i want to check the fluorescence by FACS. How much micro moles of peptide-tamra would i...

07 August 2014 3,998 3 View

How do you label cell penetration peptide?

I want to label my cell penetration peptide with low molecular weight RED fluorophore. I will analyze my samples with FACS. I am also curious about which terminal of peptide is should label such...

06 July 2014 7,563 1 View

How do you couple the quinoline functional group to the c terminal of a peptide?

I am interested in coupling quinoline as a functional group to the c terminal of my peptide with the idea that quinoline can help in endosomal escape. Please suggest some chemistry for the...

06 July 2014 3,831 7 View

Is it possible to form an amide bond between two proteins by amino-carboxyl group interaction?

I am looking for making an interaction of two proteins invitro using this strategy. Please let me know about some couplers or any alternate way of coupling two proteins or peptides using this...

04 May 2014 6,671 9 View

Can I have a protocol for eluting my HIS tagged protein using EDTA?

04 May 2014 6,839 6 View

Does anybody have experience with HSP-90 alpha?

What exactly is the role of HSP-90 in extracellular environment of the cell? I am wondering whether hsp90 is involved in the translocation of the client protein from outside to inside of the cell....

04 May 2014 6,170 2 View

Why is my HIS tagged peptide-tomato recombinant protein not released from nickle beads in the column after adding elution buffer?

The other fusion proteins are eluted well. But there is something wrong with only one protein. It is expressed well in 500 ml LB with 0.2mM IPTG, overnight incubation at 20C and 150rpm. The...

04 May 2014 3,256 10 View

Can somebody help me find some tools for in-silco membrane-peptide interaction?

I am interested in finding some bioinformatics tools to study how peptides could interact with the cell membrane and what the outcome of this interaction could be. Please help in this regard.

04 May 2014 814 1 View

How can I evaluate the penetration capability of a cell penetrating peptide in silico?

I want to construct my own cell penetrating peptide for which I want to evaluate their capability of penetrating the plasma membrane. Please share some authenticated and cited online tools which...

04 May 2014 8,899 5 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View