How do I measure zeta size of a complex containing calcium chloride- and tamra- labelled cationic plasmid dna using zetasizer?

I want to optimize the parameters needed to obtain the exact size of the nanoparticles?

31 December 2014 8,806 0 View

Is anyone familiar with plasmid DNA staining with SYBR green?

I want to stain plasmid DNA with sybr green so that I could detect it after cellular delivery. Is it possible? If yes how would I use sybr green?

11 December 2014 5,577 3 View

Is there anyone who has dealt with TAMRA detection by flow cytometry?

My peptide is labelled with TAMRA. I want to deliver that TAMRA coupled peptide to the cell lines and then i want to check the fluorescence by FACS. How much micro moles of peptide-tamra would i...

07 August 2014 3,944 3 View

How do you couple the quinoline functional group to the c terminal of a peptide?

I am interested in coupling quinoline as a functional group to the c terminal of my peptide with the idea that quinoline can help in endosomal escape. Please suggest some chemistry for the...

06 July 2014 3,784 7 View

Is it possible to form an amide bond between two proteins by amino-carboxyl group interaction?

I am looking for making an interaction of two proteins invitro using this strategy. Please let me know about some couplers or any alternate way of coupling two proteins or peptides using this...

04 May 2014 6,627 9 View

Can I have a protocol for eluting my HIS tagged protein using EDTA?

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04 May 2014 6,782 6 View

Does anybody have experience with HSP-90 alpha?

What exactly is the role of HSP-90 in extracellular environment of the cell? I am wondering whether hsp90 is involved in the translocation of the client protein from outside to inside of the cell....

04 May 2014 6,117 2 View

Why is my HIS tagged peptide-tomato recombinant protein not released from nickle beads in the column after adding elution buffer?

The other fusion proteins are eluted well. But there is something wrong with only one protein. It is expressed well in 500 ml LB with 0.2mM IPTG, overnight incubation at 20C and 150rpm. The...

04 May 2014 3,210 10 View

Can somebody help me find some tools for in-silco membrane-peptide interaction?

I am interested in finding some bioinformatics tools to study how peptides could interact with the cell membrane and what the outcome of this interaction could be. Please help in this regard.

04 May 2014 763 1 View

How can I evaluate the penetration capability of a cell penetrating peptide in silico?

I want to construct my own cell penetrating peptide for which I want to evaluate their capability of penetrating the plasma membrane. Please share some authenticated and cited online tools which...

04 May 2014 8,855 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View