Dear All,
I want to measure the Mn2+ in HCT-15 cells after treated with ic50 values of drugs.Is anyone done this experiment before by using spectrofluri meter and also what is buffer composition for this assay.
Hi to All, I am working on synergistic effect of two drugs,i want to check these drugs effect in insilico method.how can i check these effect in insilico method.
09 October 2016 7,526 1 View
i am working on anticancer drugs that may cause oxidative stress in cancer and normal cells.my interest is, treatment of that drug to RBC cells in invitro and to check the ROS generation on the...
05 June 2016 4,961 6 View
Dear researchers, I am working on cancer cell and human RBC cells.Testing cell viability,we using different assay like MTT and trypan blue.Now we have spectrofluorimeter.Can...
05 June 2016 5,122 1 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.
06 August 2024 8,848 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View