i am working on anticancer drugs that may cause oxidative stress in cancer and normal cells.my interest is, treatment of that drug to RBC cells in invitro and to check the ROS generation on the cells.
Glutathione and its oxidized forms (i.e. GSSG and PSSG) are good biomarkers of ROS presence/increase (if correctly measured).
https://www.researchgate.net/publication/7090127_Oxidized_Forms_of_Glutathione_in_Peripheral_Blood_as_Biomarkers_of_Oxidative_Stress
https://www.researchgate.net/publication/255714813_Analysis_of_GSH_and_GSSG_after_derivatization_with_N-ethylmaleimide
Article Oxidized Forms of Glutathione in Peripheral Blood as Biomark...
Article Analysis of GSH and GSSG after derivatization with N-ethylmaleimide
Dear Christina Erna Maria Krämer,
We dont have flowcytometer but we have flurocent spectrophoto meter.here,my question is ,the sample preparation for ROS is same like flow cytometer or different.can you please help me in this.
A combo of tests can be done..try the dcf-da assay alongwith catalase & SOD activity (kits) as well as immunoblots for same..then you may further add any of the tests for oxidized GSSG level.
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